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Effect of Introns and AT-rich Sequences on Expression of the Bacterial Hygromycin B Resistance Gene in the Basidiomycete Schizophyllum Commune

Overview
Journal Appl Environ Microbiol
Specialties Environmental Health
Microbiology
Date 2001 Jan 3
PMID 11133486
Citations 21
Authors
K Scholtmeijer
H A Wosten
J Springer
J G Wessels
Affiliations
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Abstract

Previously, it was shown that introns are required for efficient mRNA accumulation in Schizophyllum commune and that the presence of AT-rich sequences in the coding region of genes can result in truncation of transcripts in this homobasidiomycete. Here we show that intron-dependent mRNA accumulation and truncation of transcripts are two independent events that both affect expression of the bacterial hygromycin B resistance gene in S. commune.

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