Lectin Analysis of Human Immunoglobulin G N-glycan Sialylation

Overview

Journal Glycoconj J

Publisher Springer

Specialties Biochemistry
Endocrinology

Date 2001 Jan 2

PMID 11133020

Citations 15

Authors

M Dalziel

I McFarlane

J S Axford

Affiliations

Soon will be listed here.

Abstract

The lectins Sambucus nigra agglutinin (SNA) and Ricinus communis agglutinin (RCA), specific for alpha2,6 linked sialylation, and terminal galactose respectively were used to study the occurrence, linkage and distribution of human immunoglobulin G (IgG) sialylation. SNA was shown to bind N-glycan alpha2,6-linked sialic acid only. Sialidase analysis confirmed that this is the dominant, if not exclusive linkage. Total IgG sialylation was estimated at 1.0 microg SA/mg IgG (or about 0.5 mole per mole) using a biochemical sialic acid assay. SNA displayed strong binding to the IgG Fab fragment in both its native and denatured state. In contrast, SNA failed to bind the IgG Fc fragment in its native form, but displayed strong binding after the Fc was denatured. This allowed the construction of quantitative assays capable of measuring both IgG Fab and Fc alpha2,6-sialylation without the need for enzymatic peptide digestion.

Citing Articles

The role of antibody glycosylation in autoimmune and alloimmune kidney diseases.

Beyze A, Larroque C, le Quintrec M Nat Rev Nephrol. 2024; 20(10):672-689.

PMID: 38961307 DOI: 10.1038/s41581-024-00850-0.

Gemcitabine alters sialic acid binding of the glycocalyx and induces inflammatory cytokine production in cultured endothelial cells.

Gunji M, Sawa C, Akiyama M, Mukai S, Takaki T, Kang D Med Mol Morphol. 2023; 56(2):128-137.

PMID: 36622466 PMC: 9828377. DOI: 10.1007/s00795-022-00347-4.

Capillary (Gel) Electrophoresis-Based Methods for Immunoglobulin (G) Glycosylation Analysis.

Cajic S, Hennig R, Burock R, Rapp E Exp Suppl. 2021; 112:137-172.

PMID: 34687009 DOI: 10.1007/978-3-030-76912-3_4.

Lectin-Based Method for Deciphering Human Milk IgG Sialylation.

Lis-Kuberka J, Krolak-Olejnik B, Berghausen-Mazur M, Orczyk-Pawilowicz M Molecules. 2019; 24(20).

PMID: 31652515 PMC: 6832633. DOI: 10.3390/molecules24203797.

Lectin bio-layer interferometry for assessing product quality of Fc- glycosylated immunoglobulin G.

Wallner J, Sissolak B, Sommeregger W, Lingg N, Striedner G, Vorauer-Uhl K Biotechnol Prog. 2019; 35(5):e2864.

PMID: 31180180 PMC: 6852021. DOI: 10.1002/btpr.2864.

References

Tsuji S, Datta A, Paulson J . Systematic nomenclature for sialyltransferases. Glycobiology. 1996; 6(7):v-vii. DOI: 10.1093/glycob/6.7.647. View

Savvidou G, Klein M, Grey A, DORRINGTON K, CARVER J . Possible role for peptide-oligosaccharide interactions in differential oligosaccharide processing at asparagine-107 of the light chain and asparagine-297 of the heavy chain in a monoclonal IgG1 kappa. Biochemistry. 1984; 23(16):3736-40. DOI: 10.1021/bi00311a026. View

Rademacher T . Network theory of glycosylation--etiologic and pathogenic implications of changes in IgG glycoform levels in autoimmunity. Semin Cell Biol. 1991; 2(5):327-37. View

FISCHER E, BROSSMER R . Sialic acid-binding lectins: submolecular specificity and interaction with sialoglycoproteins and tumour cells. Glycoconj J. 1995; 12(5):707-13. DOI: 10.1007/BF00731268. View

Routier F, Davies M, Bergemann K, Hounsell E . The glycosylation pattern of humanized IgGI antibody (D1.3) expressed in CHO cells. Glycoconj J. 1997; 14(2):201-7. DOI: 10.1023/a:1018589704981. View

Youinou P, Pennec Y, Casburn-Budd R, Dueymes M, Letoux G, Lamour A . Galactose terminating oligosaccharides of IgG in patients with primary Sjögren's syndrome. J Autoimmun. 1992; 5(3):393-400. DOI: 10.1016/0896-8411(92)90151-f. View

Sumar N, Isenberg D, Bodman K, Soltys A, Young A, Leak A . Reduction in IgG galactose in juvenile and adult onset rheumatoid arthritis measured by a lectin binding method and its relation to rheumatoid factor. Ann Rheum Dis. 1991; 50(9):607-10. PMC: 1004502. DOI: 10.1136/ard.50.9.607. View

Skoza L, Mohos S . Stable thiobarbituric acid chromophore with dimethyl sulphoxide. Application to sialic acid assay in analytical de-O-acetylation. Biochem J. 1976; 159(3):457-62. PMC: 1164141. DOI: 10.1042/bj1590457. View

Pekelharing J, Hepp E, Kamerling J, Gerwig G, Leijnse B . Alterations in carbohydrate composition of serum IgG from patients with rheumatoid arthritis and from pregnant women. Ann Rheum Dis. 1988; 47(2):91-5. PMC: 1003459. DOI: 10.1136/ard.47.2.91. View

10.

Stanley P, Raju T, Bhaumik M . CHO cells provide access to novel N-glycans and developmentally regulated glycosyltransferases. Glycobiology. 1996; 6(7):695-9. DOI: 10.1093/glycob/6.7.695. View

11.

Wang W, Cummings R . The immobilized leukoagglutinin from the seeds of Maackia amurensis binds with high affinity to complex-type Asn-linked oligosaccharides containing terminal sialic acid-linked alpha-2,3 to penultimate galactose residues. J Biol Chem. 1988; 263(10):4576-85. View

12.

Augener W, Brittinger G, Schiphorst W, van den Eijnden D . Activities and specificities of N-acetylneuraminyltransferases in leukemic cells. Haematol Blood Transfus. 1983; 28:135-8. DOI: 10.1007/978-3-642-68761-7_30. View

13.

Iobst S, Drickamer K . Selective sugar binding to the carbohydrate recognition domains of the rat hepatic and macrophage asialoglycoprotein receptors. J Biol Chem. 1996; 271(12):6686-93. DOI: 10.1074/jbc.271.12.6686. View

14.

Nemansky M, Schiphorst W, van den Eijnden D . Branching and elongation with lactosaminoglycan chains of N-linked oligosaccharides result in a shift toward termination with alpha 2-->3-linked rather than with alpha 2-->6-linked sialic acid residues. FEBS Lett. 1995; 363(3):280-4. DOI: 10.1016/0014-5793(95)00336-8. View

15.

Shibuya N, Goldstein I, Broekaert W, Nsimba-Lubaki M, Peeters B, Peumans W . The elderberry (Sambucus nigra L.) bark lectin recognizes the Neu5Ac(alpha 2-6)Gal/GalNAc sequence. J Biol Chem. 1987; 262(4):1596-601. View

16.

Bond A, Jones M, Hay F . Human IgG preparations isolated by ion-exchange or protein G affinity chromatography differ in their glycosylation profiles. J Immunol Methods. 1993; 166(1):27-33. DOI: 10.1016/0022-1759(93)90325-2. View

17.

Boyd P, Lines A, Patel A . The effect of the removal of sialic acid, galactose and total carbohydrate on the functional activity of Campath-1H. Mol Immunol. 1995; 32(17-18):1311-8. DOI: 10.1016/0161-5890(95)00118-2. View

18.

Schauer R . Biosynthesis and function of N- and O-substituted sialic acids. Glycobiology. 1991; 1(5):449-52. DOI: 10.1093/glycob/1.5.449. View

19.

Rudd P, Leatherbarrow R, Rademacher T, Dwek R . Diversification of the IgG molecule by oligosaccharides. Mol Immunol. 1991; 28(12):1369-78. DOI: 10.1016/0161-5890(91)90039-m. View

20.

Goodarzi M, Axford J, Varanasi S, Alavi A, Cunnane G, FitzGerald O . Sialyl Lewis(x) expression on IgG in rheumatoid arthritis and other arthritic conditions: a preliminary study. Glycoconj J. 1999; 15(12):1149-54. DOI: 10.1023/a:1006920007227. View