Kinetic Studies of Imidazole and Its Methyl Derivative Binding to Metmyoglobin: Effects of Substitute Methyl on the Binding Affinity

Overview

Journal Biometals

Specialty Biochemistry

Date 2000 Dec 29

PMID 11127899

Citations 3

Authors

C Qian

Y Yao

Y Wu

W Tang

Affiliations

Soon will be listed here.

Abstract

The binding of imidazole and imidazole derivatives (4- and 2-MeIm) to the metMb was studied by 1H NMR spectroscopy to elucidate the effects of different methyl substitution positions on the affinity and kinetics of binding to metMb. These exogenous ligands can form stable complexes with metMb except 2-MeIm. Kinetics and equilibrium data for the binding of imidazole and 4-MeIm to metMb have been obtained by 2D EXSY, and the reasons for the different affinity are also discussed.

Citing Articles

Binding of imidazole, 1-methylimidazole and 4-nitroimidazole to yeast cytochrome c peroxidase (CcP) and the distal histidine mutant, CcP(H52L).

Erman J, Chinchilla D, Studer J, Vitello L Biochim Biophys Acta. 2015; 1854(8):869-81.

PMID: 25907133 PMC: 4457651. DOI: 10.1016/j.bbapap.2015.04.013.

Kinetic and equilibrium studies of acrylonitrile binding to cytochrome c peroxidase and oxidation of acrylonitrile by cytochrome c peroxidase compound I.

Chinchilla D, Kilheeney H, Vitello L, Erman J Biochem Biophys Res Commun. 2013; 443(1):200-4.

PMID: 24291498 PMC: 3896955. DOI: 10.1016/j.bbrc.2013.11.084.

Modeling proline ligation in the heme-dependent CO sensor, CooA, using small-molecule analogs.

Pinkert J, Clark R, Burstyn J J Biol Inorg Chem. 2006; 11(5):642-50.

PMID: 16724227 DOI: 10.1007/s00775-006-0115-8.