The Cellular Receptor to Human Rhinovirus 2 Binds Around the 5-fold Axis and Not in the Canyon: a Structural View

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2000 Dec 2

PMID 11101504

Citations 57

Authors

E A Hewat

E Neumann

J F Conway

R Moser

B Ronacher

T C Marlovits

D Blaas

Affiliations

Soon will be listed here.

Abstract

Human rhinovirus serotype 2 (HRV2) belongs to the minor group of HRVs that bind to members of the LDL-receptor family including the very low density lipoprotein (VLDL)-receptor (VLDL-R). We have determined the structures of the complex between HRV2 and soluble fragments of the VLDL-R to 15 A resolution by cryo-electron microscopy. The receptor fragments, which include the first three ligand-binding repeats of the VLDL-R (V1-3), bind to the small star-shaped dome on the icosahedral 5-fold axis. This is in sharp contrast to the major group of HRVs where the receptor site for ICAM-1 is located at the base of a depression around each 5-fold axis. Homology models of the three domains of V1-3 were used to explore the virus-receptor interaction. The footprint of VLDL-R on the viral surface covers the BC- and HI-loops on VP1.

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References

Okun V, Ronacher B, Blaas D, Kenndler E . Analysis of common cold virus (human rhinovirus serotype 2) by capillary zone electrophoresis: the problem of peak identification. Anal Chem. 1999; 71(10):2028-32. DOI: 10.1021/ac981324x. View

Saxton W, Baumeister W . The correlation averaging of a regularly arranged bacterial cell envelope protein. J Microsc. 1982; 127(Pt 2):127-38. DOI: 10.1111/j.1365-2818.1982.tb00405.x. View

Conway J, Steven A . Methods for reconstructing density maps of "single" particles from cryoelectron micrographs to subnanometer resolution. J Struct Biol. 1999; 128(1):106-18. DOI: 10.1006/jsbi.1999.4168. View

Marlovits T, Abrahamsberg C, Blaas D . Very-low-density lipoprotein receptor fragment shed from HeLa cells inhibits human rhinovirus infection. J Virol. 1998; 72(12):10246-50. PMC: 110607. DOI: 10.1128/JVI.72.12.10246-10250.1998. View

Gruenberger M, Pevear D, Diana G, Kuechler E, Blaas D . Stabilization of human rhinovirus serotype 2 against pH-induced conformational change by antiviral compounds. J Gen Virol. 1991; 72 ( Pt 2):431-3. DOI: 10.1099/0022-1317-72-2-431. View

Colonno R, Condra J, Mizutani S, Callahan P, Davies M, Murcko M . Evidence for the direct involvement of the rhinovirus canyon in receptor binding. Proc Natl Acad Sci U S A. 1988; 85(15):5449-53. PMC: 281774. DOI: 10.1073/pnas.85.15.5449. View

Pevear D, Fancher M, Felock P, Rossmann M, Miller M, Diana G . Conformational change in the floor of the human rhinovirus canyon blocks adsorption to HeLa cell receptors. J Virol. 1989; 63(5):2002-7. PMC: 250615. DOI: 10.1128/JVI.63.5.2002-2007.1989. View

Colston E, Racaniello V . Soluble receptor-resistant poliovirus mutants identify surface and internal capsid residues that control interaction with the cell receptor. EMBO J. 1994; 13(24):5855-62. PMC: 395560. DOI: 10.1002/j.1460-2075.1994.tb06930.x. View

Fuller S, Butcher S, Cheng R, Baker T . Three-dimensional reconstruction of icosahedral particles--the uncommon line. J Struct Biol. 1996; 116(1):48-55. DOI: 10.1006/jsbi.1996.0009. View

10.

Kolatkar P, Bella J, Olson N, Bator C, Baker T, Rossmann M . Structural studies of two rhinovirus serotypes complexed with fragments of their cellular receptor. EMBO J. 1999; 18(22):6249-59. PMC: 1171688. DOI: 10.1093/emboj/18.22.6249. View

11.

Kim S, Boege U, Krishnaswamy S, Minor I, Smith T, Luo M . Conformational variability of a picornavirus capsid: pH-dependent structural changes of Mengo virus related to its host receptor attachment site and disassembly. Virology. 1990; 175(1):176-90. DOI: 10.1016/0042-6822(90)90198-z. View

12.

North C, Blacklow S . Structural independence of ligand-binding modules five and six of the LDL receptor. Biochemistry. 1999; 38(13):3926-35. DOI: 10.1021/bi9821622. View

13.

Brown M, Goldstein J . A receptor-mediated pathway for cholesterol homeostasis. Science. 1986; 232(4746):34-47. DOI: 10.1126/science.3513311. View

14.

Rossmann M, Arnold E, Erickson J, Frankenberger E, Griffith J, Hecht H . Structure of a human common cold virus and functional relationship to other picornaviruses. Nature. 1985; 317(6033):145-53. DOI: 10.1038/317145a0. View

15.

Dolmer K, Huang W, Gettins P . NMR solution structure of complement-like repeat CR3 from the low density lipoprotein receptor-related protein. Evidence for specific binding to the receptor binding domain of human alpha(2)-macroglobulin. J Biol Chem. 2000; 275(5):3264-9. DOI: 10.1074/jbc.275.5.3264. View

16.

Neubauer C, Frasel L, Kuechler E, Blaas D . Mechanism of entry of human rhinovirus 2 into HeLa cells. Virology. 1987; 158(1):255-8. DOI: 10.1016/0042-6822(87)90264-9. View

17.

Chapman M . Mapping the surface properties of macromolecules. Protein Sci. 1993; 2(3):459-69. PMC: 2142375. DOI: 10.1002/pro.5560020318. View

18.

Smith T, Chase E, Schmidt T, Olson N, Baker T . Neutralizing antibody to human rhinovirus 14 penetrates the receptor-binding canyon. Nature. 1996; 383(6598):350-4. PMC: 4167671. DOI: 10.1038/383350a0. View

19.

Fuller S . The T=4 envelope of Sindbis virus is organized by interactions with a complementary T=3 capsid. Cell. 1987; 48(6):923-34. DOI: 10.1016/0092-8674(87)90701-x. View

20.

Jeon H, Shipley G . Vesicle-reconstituted low density lipoprotein receptor. Visualization by cryoelectron microscopy. J Biol Chem. 2000; 275(39):30458-64. DOI: 10.1074/jbc.M002583200. View