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Analytical Isoelectric Focusing of R Factor-determined Beta-lactamases: Correlation with Plasmid Compatibility

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1976 Feb 1
PMID 1107329
Citations 74
Authors
M Matthew
R W Hedges
Affiliations
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Abstract

R factor-determined beta-lactamases have been investigated by analytical isoelectric focusing. The enzymes such as those specified by the R6K and RP4 plasmids (TEM-type enzymes) are notably homogenous in biochemical tests (Hedges et al., 1974), but two subclasses can be distinguished by isoelectric focusing. Three subclasses can be distinguished among the oxacillin-hydrolyzing enzymes, in good agreement with the classification based upon biochemical characteristics (Dale and Smith, 1974). The TEM-type beta-lactamases are promiscuously distributed among plasmids of a wide variety of compatibility groups, whereas the various oxacillin-hydrolyzing enzymes show some degree of correlation with compatibility.

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