Cytochrome Abnormalities and Cyanide-resistant Respiration in Extranuclear Mutants of Aspergillus Nidulans

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1976 Feb 1

PMID 1107321

Citations 6

Authors

G Turner

R T Rowlands

Affiliations

Soon will be listed here.

Abstract

The cytochrome spectra of two extranuclear mutants of Aspergillus nidulans and the double-mutant recombinant formed from them have been examined both at room temperature and at the temperature of liquid N2 and compared with those of the wild-type strain. The oligomycin-resistant, slow growing mutant contained an increased amount of cytochrome c without any loss of cytochromes b and a,a3. The cold-sensitive mutant, apparently normal when grown at 37 C, showed an increased amount of cytochrome c and a partial loss of cytochromes b and a,a3 when grown at 20 C. A combination of these effects was observed in the double-mutant recombinant. Cyanide-resistant respiration was present in both mutant strains and in the recombinant at much higher levels than in the wild-type strain. In the oligomycin-resistant mutant, this was usually present together with cyanide-sensitive respiration, whereas in the cold-sensitive mutant and recombinant grown at 20 C cyanide-resistant approached 100%. Inhibitor and growth yield studies indicated that the cyanide-resistant pathway was not used by the cold-sensitive mutant during growth at 20 C.

Citing Articles

Mitochondrial genes in Podospora anserina: recombination and linkage.

Belcour L, Begel O Mol Gen Genet. 1977; 153(1):11-21.

PMID: 887068 DOI: 10.1007/BF01035991.

Three-marker extranuclear mitochondrial crosses in Aspergillus nidulans.

Rowlands R, Turner G Mol Gen Genet. 1975; 141(1):69-79.

PMID: 765725 DOI: 10.1007/BF00332379.

Extranuclear mutant of Schizophyllum commune.

Nesvera J Folia Microbiol (Praha). 1978; 23(5):379-84.

PMID: 568101 DOI: 10.1007/BF02876439.

Extranuclear recombination in Aspergillus nidulans: closely-linked multiple chloramphenicol- and oligomycin-resistance loci.

Lazarus C, Turner G Mol Gen Genet. 1977; 156(3):303-11.

PMID: 340908 DOI: 10.1007/BF00267186.

Physiological consequences of mitochondrial antibiotic-resistant mutations in Paramecium: growth-rates, cytochromic defects and cyanide-insensitive respiration of mutant and erythromycin-treated wild-type strains.

Adoutte A, Doussiere J Mol Gen Genet. 1978; 161(2):121-34.

PMID: 209305

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