Tolbutamide-induced Changes of the DNA, Protein and Insulin Content and the Secretory Activity of Isolated Rat Pancreatic Islets

Overview

Journal Diabetologia

Specialty Endocrinology

Date 1975 Aug 1

PMID 1107120

Citations 4

Authors

P Schauder

H Frerichs

Affiliations

Soon will be listed here.

Abstract

Following prolonged administration of tolbutamide the DNA- and protein content per islet was enhanced but the IRI content per islet was diminished. Glucose-induced (2.0, 8.0 or 16.6 mM) and leucine-induced (12.5 or 25.0 mM) IRI release from isolated islets, as well as 14C02-production from U-14C glucose, were decreased. Theophylline (5.0 mM) restored the glucose sensitivity of the islets towards normal. The results indicate that tolbutamide-induced islet cell hyperplasia does not entail islet hyperfunction, as previously thought. Decreased IRI release may partially be explained by a tolbutamide-induced alteration of the adenylate cyclase/phosphodiesterase system of the B-cell.

Citing Articles

Long-term effects of glibenclamide on the insulin production, oxidative metabolism and quantitative ultrastructure of mouse pancreatic islets maintained in tissue culture at different glucose concentrations.

Borg L, Andersson A Acta Diabetol Lat. 1981; 18(1):65-83.

PMID: 6782787 DOI: 10.1007/BF02056108.

Insulin secretion and (pro)insulin biosynthesis of cultured mice islets after glibenclamide loading in vitro.

Ziegler B, Wilke B, Ziegler M, Schmidt S, Zuhlke H Experientia. 1980; 36(4):467-8.

PMID: 6769698 DOI: 10.1007/BF01975151.

Plasma insulin and glucose levels in maturity onset diabetics treated with chlorpropamide.

Shenfield G, Logan A, SHIRLING D, Baird J Diabetologia. 1977; 13(4):367-71.

PMID: 913926 DOI: 10.1007/BF01223280.

Cytochalasin B: evidence for a membrane-directed action in B-cells from rat pancreatic islets.

Schauder P, Frerichs H Pflugers Arch. 1976; 364(1):95-8.

PMID: 785375 DOI: 10.1007/BF01062918.

References

Gerner R, Lage-Stehr J, Tjioe T, Wacker A . [Isolation of Langerhans' islets from rat pancreas]. Hoppe Seylers Z Physiol Chem. 1970; 351(3):309-12. View

DUNBAR J, FOA P . An inhibitory effect of tolbutamide and glibenclamide (glyburide) on the pancreatic islets of normal animals. Diabetologia. 1974; 10(1):27-35. DOI: 10.1007/BF00421411. View

GEPTS W . [Histological study of the effect of the hypoglycemic sulphamides on the islands of Langerhans of the rat; study of the effect of BZ-55 treatment associated with cortisone]. Ann Endocrinol (Paris). 1957; 18(2):204-17. View

Tjioe T, Wacker A . [The effect of oral antidiabetics on the biosynthesis of proinsulin in the rat]. Arzneimittelforschung. 1972; 22(11):1890-2. View

Kracht J, VON HOLT C, VON HOLT L . [Morphological findings on the mode of action of oral antidiabetics]. Endokrinologie. 1957; 34(3):129-46. View

VOLK B, Lazarus S . B CELL HYPERFUNCTION AFTER LONG-TERM SULFONYLUREA TREATMENT. Arch Pathol. 1964; 78:115-26. View

Schauder P, Frerichs H . Cytochalasin B: inhibition of glucose-induced insulin release from isolated rat pancreatic islets. Diabetologia. 1974; 10(1):85-7. DOI: 10.1007/BF00421418. View

Johansen K, Orskov H . The stimulatory effect on insulin secretion in long-term tolbutamide treatment. Immunological determinations of urinary- and serum insulin. Acta Endocrinol (Copenh). 1972; 71(4):709-15. DOI: 10.1530/acta.0.0710709. View

Georg R, SUSSMAN K, Leitner J, Kirsch W . Inhibition of glucose and tolbutamide-induced insulin release by iodoacetate and antimycin A. Endocrinology. 1971; 89(1):169-76. DOI: 10.1210/endo-89-1-169. View

10.

Kuo W, Hodgins D, Kuo J . Adenylate cyclase in islets of Langerhans. Isolation of islets and regulation of adenylate cyclase activity by various hormones and agents. J Biol Chem. 1973; 248(8):2705-11. View

11.

Hellman B, Idahl L, Danielsson A . Adenosine triphosphate levels of mammalian pancreatic B cells after stimulation with glucose and hypoglycemic sulfonylureas. Diabetes. 1969; 18(8):509-16. DOI: 10.2337/diab.18.8.509. View

12.

Bellens R, Christophe J, GEPTS W . [Experimental study of the effect of BZ 55 on normal and alloxan-treated rats; morphological changes, especially pancreatic changes]. Ann Endocrinol (Paris). 1956; 17(2):278-90. View

13.

CHU P, Conway M, Krouse H, GOODNER C . The pattern of rsponse of plasma insulin and glucose to meals and fasting during chlorpropamide therapy. Ann Intern Med. 1968; 68(4):757-69. DOI: 10.7326/0003-4819-68-4-757. View

14.

Reaven G, Dray J . Effect of chlorpropamide on serum glucose and immunoreactive insulin concentrations in patients with maturity-onset diabetes mellitus. Diabetes. 1967; 16(7):487-92. DOI: 10.2337/diab.16.7.487. View

15.

Malaisse W, Malaisse-Lagae F, Mayhew D . A possible role for the adenylcyclase system in insulin secretion. J Clin Invest. 1967; 46(11):1724-34. PMC: 292923. DOI: 10.1172/JCI105663. View

16.

Gill J . Insulin release by isolated pancreatic islets of the mouse incubated in vitro. Diabetologia. 1969; 5(2):61-6. DOI: 10.1007/BF01211999. View

17.

Melani F, DITSCHUNEIT H, Bartelt K, Friedrich H, Pfeiffer E . [On radio-immunologic determination of insulin in blood]. Klin Wochenschr. 1965; 43(18):1000-7. DOI: 10.1007/BF01747863. View

18.

Loreti L, Sugase T, FOA P . Diurnal variations of serum insulin, total glucagon, cortisol, glucose and free fatty acids in normal and diabetic subjects before and after treatment with chlorpropamide. Horm Res. 1974; 5(5):278-92. DOI: 10.1159/000178641. View

19.

Morris G, Korner A . The effect of glucose on insulin biosynthesis by isolated islets of Langerhans of the rat. Biochim Biophys Acta. 1970; 208(3):404-13. DOI: 10.1016/0304-4165(70)90213-8. View

20.

Westman S, HELLERSTROM C . The metabolism of the pancreatic beta-cells of mice in the presence of hypoglycaemic sulphonylureas. Postgrad Med J. 1970; :Suppl:28-31. View