RecA-independent Ectopic Transposition in Vivo of a Bacterial Group II Intron

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2000 Nov 1

PMID 11058141

Citations 19

Authors

F Martinez-Abarca

N Toro

Affiliations

Soon will be listed here.

Abstract

RmInt1 is a group II intron of Sinorhizobium meliloti which was initially found within the insertion sequence ISRm2011-2. Although the RmInt1 intron-encoded protein lacks a recognizable endonuclease domain, it is able to mediate insertion of RmInt1 at an intron-specific location in intronless ISRm2011-2 recipient DNA, a phenomenon termed homing. Here we have characterized three additional insertion sites of RmInt1 in the genome of S.meliloti. Two of these sites are within IS elements closely related to ISRm2011-2, which appear to form a characteristic group within the IS630-Tc1 family. The third site is in the oxi1 gene, which encodes a putative oxide reductase. The newly identified integration sites contain conserved intron-binding site (IBS1 and IBS2) and delta' sequences (14 bp). The RNA of the intron-containing oxi1 gene is able to splice and the oxi1 site is a DNA target for RmInt1 transposition in vivo. Ectopic transposition of RmInt1 into the oxi1 gene occurs at 20-fold lower efficiency than into the homing site (ISRm2011-2) and is independent of the major RecA recombination pathway. The possibility that transposition of RmInt1 to the oxi1 site occurs by reverse splicing into DNA is discussed.

Citing Articles

Contribution of Mobile Group II Introns to Genome Evolution.

Toro N, Martinez-Abarca F, Molina-Sanchez M, Garcia-Rodriguez F, Nisa-Martinez R Front Microbiol. 2018; 9:627.

PMID: 29670598 PMC: 5894124. DOI: 10.3389/fmicb.2018.00627.

Inactivation of group II intron RmInt1 in the Sinorhizobium meliloti genome.

Molina-Sanchez M, Toro N Sci Rep. 2015; 5:12036.

PMID: 26156864 PMC: 4496777. DOI: 10.1038/srep12036.

Mobile Bacterial Group II Introns at the Crux of Eukaryotic Evolution.

Lambowitz A, Belfort M Microbiol Spectr. 2015; 3(1):MDNA3-0050-2014.

PMID: 26104554 PMC: 4394904. DOI: 10.1128/microbiolspec.MDNA3-0050-2014.

Insights into the strategies used by related group II introns to adapt successfully for the colonisation of a bacterial genome.

Martinez-Rodriguez L, Garcia-Rodriguez F, Molina-Sanchez M, Toro N, Martinez-Abarca F RNA Biol. 2014; 11(8):1061-71.

PMID: 25482895 PMC: 4615759. DOI: 10.4161/rna.32092.

Alternative splicing of a group II intron in a surface layer protein gene in Clostridium tetani.

McNeil B, Simon D, Zimmerly S Nucleic Acids Res. 2013; 42(3):1959-69.

PMID: 24214997 PMC: 3919590. DOI: 10.1093/nar/gkt1053.

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