Unmodified Calcium Concentrations in Tumour Necrosis Factor Receptor Subtype-mediated Apoptotic Cell Death

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2000 Oct 31

PMID 11055543

Citations 5

Authors

S M McFarlane

H M Anderson

S J Tucker

O J Jupp

D J MacEwan

Affiliations

Soon will be listed here.

Abstract

Tumour necrosis factor-alpha (TNF) receptors mediate a variety of effects dependent on cell type. A role for Ca2+ in TNF-induced death remains uncertain. Here we investigated restricting intracellular/extracellular Ca2+ in HeLa epithelial carcinoma cells expressing low and high levels of p75TNFR receptor subtype and KYM-1 rhabdomyosarcoma cells, models of rapid TNF-induced apoptosis. Ca2+ -chelators EGTA and BAPTA-AM as well as microsomal Ca2+ -ATPase inhibitor thapsigargin, did not alter TNF-induced death. TNF was also unable to alter resting [Ca2+]i levels which remained < 200 nM even during times when these cells were undergoing apoptotic cell death. These findings indicate no role for modulated Ca2+ concentrations in TNF-induced apoptotic cell death.

Citing Articles

Alix and ALG-2 are involved in tumor necrosis factor receptor 1-induced cell death.

Mahul-Mellier A, Strappazzon F, Petiot A, Chatellard-Causse C, Torch S, Blot B J Biol Chem. 2008; 283(50):34954-65.

PMID: 18936101 PMC: 3259881. DOI: 10.1074/jbc.M803140200.

Long-chain ceramide is a potent inhibitor of the mitochondrial permeability transition pore.

Novgorodov S, Gudz T, Obeid L J Biol Chem. 2008; 283(36):24707-17.

PMID: 18596045 PMC: 2529003. DOI: 10.1074/jbc.M801810200.

Distinct regulation of cytosolic phospholipase A2 phosphorylation, translocation, proteolysis and activation by tumour necrosis factor-receptor subtypes.

Jupp O, Vandenabeele P, MacEwan D Biochem J. 2003; 374(Pt 2):453-61.

PMID: 12786601 PMC: 1223607. DOI: 10.1042/BJ20030705.

TNF ligands and receptors--a matter of life and death.

MacEwan D Br J Pharmacol. 2002; 135(4):855-75.

PMID: 11861313 PMC: 1573213. DOI: 10.1038/sj.bjp.0704549.

Adenovirus E3-6.7K maintains calcium homeostasis and prevents apoptosis and arachidonic acid release.

Moise A, Grant J, Vitalis T, Jefferies W J Virol. 2002; 76(4):1578-87.

PMID: 11799152 PMC: 135875. DOI: 10.1128/jvi.76.4.1578-1587.2002.

References

Porn-Ares M, Ares M, Orrenius S . Calcium signalling and the regulation of apoptosis. Toxicol In Vitro. 2010; 12(5):539-43. DOI: 10.1016/s0887-2333(98)00032-0. View

Parris J, Cobban H, Littlejohn A, MacEwan D, Nixon G . Tumour necrosis factor-alpha activates a calcium sensitization pathway in guinea-pig bronchial smooth muscle. J Physiol. 1999; 518 ( Pt 2):561-9. PMC: 2269439. DOI: 10.1111/j.1469-7793.1999.0561p.x. View

Hampton M, Vanags D, Porn-Ares M, Orrenius S . Involvement of extracellular calcium in phosphatidylserine exposure during apoptosis. FEBS Lett. 1996; 399(3):277-82. DOI: 10.1016/s0014-5793(96)01341-5. View

Beyaert R, Heyninck K, De Valck D, Boeykens F, VAN Roy F, Fiers W . Enhancement of tumor necrosis factor cytotoxicity by lithium chloride is associated with increased inositol phosphate accumulation. J Immunol. 1993; 151(1):291-300. View

Weiss T, Grell M, Hessabi B, Bourteele S, Muller G, Scheurich P . Enhancement of TNF receptor p60-mediated cytotoxicity by TNF receptor p80: requirement of the TNF receptor-associated factor-2 binding site. J Immunol. 1997; 158(5):2398-404. View

Grell M, Zimmermann G, Hulser D, Pfizenmaier K, Scheurich P . TNF receptors TR60 and TR80 can mediate apoptosis via induction of distinct signal pathways. J Immunol. 1994; 153(5):1963-72. View

Lui P, Kong S, Fung K, Lee C . The rise of nuclear and cytosolic Ca2+ can be uncoupled in HeLa cells. Pflugers Arch. 1998; 436(3):371-6. DOI: 10.1007/s004240050645. View

Kong S, Fung K, Choy Y, Lee C . Slow increase in intranuclear and cytosolic free calcium concentrations in L929 cells is important in tumour necrosis factor-alpha-mediated cell death. Oncology. 1997; 54(1):55-62. DOI: 10.1159/000227662. View

Vercammen D, Brouckaert G, Denecker G, van de Craen M, Declercq W, Fiers W . Dual signaling of the Fas receptor: initiation of both apoptotic and necrotic cell death pathways. J Exp Med. 1998; 188(5):919-30. PMC: 2213397. DOI: 10.1084/jem.188.5.919. View

10.

Cohen O, Inbal B, Kissil J, Raveh T, Berissi H, Feinstein E . DAP-kinase participates in TNF-alpha- and Fas-induced apoptosis and its function requires the death domain. J Cell Biol. 1999; 146(1):141-8. PMC: 2199731. DOI: 10.1083/jcb.146.1.141. View

11.

Branellec D, Mishal Z, Chouaib S . Modulation of TNF-mediated cell lysis in vitro: further analysis of intracellular signaling. Immunopharmacology. 1993; 26(2):147-56. DOI: 10.1016/0162-3109(93)90007-d. View

12.

Fiers W . Tumor necrosis factor. Characterization at the molecular, cellular and in vivo level. FEBS Lett. 1991; 285(2):199-212. DOI: 10.1016/0014-5793(91)80803-b. View

13.

Denecker G, Vandenabeele P, Grooten J, Penning L, Declercq W, Beyaert R . Differential role of calcium in tumour necrosis factor-mediated apoptosis and secretion of granulocyte-macrophage colony-stimulating factor in a T cell hybridoma. Cytokine. 1997; 9(9):631-8. DOI: 10.1006/cyto.1997.0218. View

14.

Porn-Ares M, Samali A, Orrenius S . Cleavage of the calpain inhibitor, calpastatin, during apoptosis. Cell Death Differ. 1999; 5(12):1028-33. DOI: 10.1038/sj.cdd.4400424. View

15.

Reid T, Louie P, Heller R . Mechanisms of tumor necrosis factor cytotoxicity and the cytotoxic signals transduced by the p75-tumor necrosis factor receptor. Circ Shock. 1994; 44(2):84-90. View

16.

Haridas V, Darnay B, Natarajan K, Heller R, Aggarwal B . Overexpression of the p80 TNF receptor leads to TNF-dependent apoptosis, nuclear factor-kappa B activation, and c-Jun kinase activation. J Immunol. 1998; 160(7):3152-62. View

17.

Heller R, Song K, Fan N, Chang D . The p70 tumor necrosis factor receptor mediates cytotoxicity. Cell. 1992; 70(1):47-56. DOI: 10.1016/0092-8674(92)90532-h. View

18.

Bellomo G, Perotti M, Taddei F, Mirabelli F, FINARDI G, Nicotera P . Tumor necrosis factor alpha induces apoptosis in mammary adenocarcinoma cells by an increase in intranuclear free Ca2+ concentration and DNA fragmentation. Cancer Res. 1992; 52(5):1342-6. View

19.

Schulze-Osthoff K, Bakker A, Vanhaesebroeck B, Beyaert R, Jacob W, Fiers W . Cytotoxic activity of tumor necrosis factor is mediated by early damage of mitochondrial functions. Evidence for the involvement of mitochondrial radical generation. J Biol Chem. 1992; 267(8):5317-23. View

20.

Vandenabeele P, Declercq W, Beyaert R, Fiers W . Two tumour necrosis factor receptors: structure and function. Trends Cell Biol. 1995; 5(10):392-9. DOI: 10.1016/s0962-8924(00)89088-1. View