The Segregation of Mitochondrial Genes in Yeast. II. Analysis of Zygote Pedigrees of Drug-resistant X Drug-sensitive Crosses

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1975 Sep 8

PMID 1102946

Citations 11

Authors

J L Forster

R A Kleese

Affiliations

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Abstract

Cytoplasmically inherited chloramphenicol- and erythromycin-resistant mutants were obtained in three unrelated and two isogenic haploid strains of yeast. The bias favoring the transmission of these resistance alleles in crosses to the isogenic strains was compared on two levels: on the population level by means of observing random diploid progeny from mass matings, and on the zygote level by zygotic pedigree analyses. The genetic basis of this bias was determined by tetrad analysis. Our results suggest that 1. an intracellular selection mechanism operates within zygotes to determine the degree of bias; 2. the selection mechanism operates differently with respect to the two loci, C and E, under consideration; and 3. the selection mechanism is controlled by a set of nuclear genes. Other models which have been suggested to explain bias are critically examined in light of our results.

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References

Linnane A, Saunders G, Gingold E, Lukins H . The biogenesis of mitochondria. V. Cytoplasmic inheritance of erythromycin resistance in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1968; 59(3):903-10. PMC: 224776. DOI: 10.1073/pnas.59.3.903. View

Kleese R, Grotbeck R, Snyder J . Recombination among three mitochondrial genes in yeast (Saccharomyces cerevisiae). J Bacteriol. 1972; 112(2):1023-5. PMC: 251519. DOI: 10.1128/jb.112.2.1023-1025.1972. View

Lukins H, Tate J, Saunders G, Linnane A . The biogenesis of mitochondria 26. Mitochondrial recombination: the segregation of parental and recombinant mitochondrial genotypes during vegetative division of yeast. Mol Gen Genet. 1973; 120(1):17-25. DOI: 10.1007/BF00332981. View

Mounolou J, Jakob H, SLONIMSKI P . Mitochondrial DNA from yeast "petite" mutants: specific changes in buoyant density corresponding to different cytoplasmic mutations. Biochem Biophys Res Commun. 1966; 24(2):218-24. DOI: 10.1016/0006-291x(66)90723-6. View

Dujon B, SLONIMSKI P, Weill L . Mitochondrial genetics IX: A model for recombination and segregation of mitochondrial genomes in saccharomyces cerevisiae. Genetics. 1974; 78(1):415-37. PMC: 1213202. DOI: 10.1093/genetics/78.1.415. View

Callen D . Segregation of mitochondially inherited antibiotic resistance genes in zygote cell lineges of Saccharomyces cerevisiae. Mol Gen Genet. 1974; 134(1):65-75. DOI: 10.1007/BF00332813. View

Wilkie D, Thomas D . Mitochondrial genetic analysis by zygote cell lineages in Saccharomyces cerevisiae. Genetics. 1973; 73(3):367-77. PMC: 1212898. DOI: 10.1093/genetics/73.3.367. View

Thomas D, Wilkie D . Recombination of mitochondrial drug-resistance factors in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1968; 30(4):368-72. DOI: 10.1016/0006-291x(68)90753-5. View

Callen D . The effect of mating type on the polarity of mitochondrial gene transmission in Saccharomyces cerevisiae. Mol Gen Genet. 1974; 128(4):321-9. DOI: 10.1007/BF00268519. View

10.

Avner P, Coen D, Dujon B, SLONIMSKI P . Mitochondrial genetics. IV. Allelism and mapping studies of oligomycin resistant mutants in S. cerevisiae. Mol Gen Genet. 1973; 125(1):9-52. DOI: 10.1007/BF00292982. View

11.

Coen D, Deutsch J, Netter P, Petrochilo E, SLONIMSKI P . Mitochondrial genetics. I. Methodology and phenomenology. Symp Soc Exp Biol. 1970; 24:449-96. View

12.

Forster J, Kleese R . The segregation of mitochondrial genes in yeast. I. Analysis of zygote pedigrees of petite X grande crosses. Mol Gen Genet. 1975; 139(4):329-39. DOI: 10.1007/BF00267973. View

13.

Perlman P, Birky Jr C . Mitochondrial genetics in Bakers' yeast: a molecular mechanism for recombinational polarity and suppressiveness. Proc Natl Acad Sci U S A. 1974; 71(11):4612-6. PMC: 433938. DOI: 10.1073/pnas.71.11.4612. View

14.

EPHRUSSI B, de Margerie-Hottinguer H, Roman H . SUPPRESSIVENESS: A NEW FACTOR IN THE GENETIC DETERMINISM OF THE SYNTHESIS OF RESPIRATORY ENZYMES IN YEAST. Proc Natl Acad Sci U S A. 1955; 41(12):1065-71. PMC: 528198. DOI: 10.1073/pnas.41.12.1065. View

15.

Rank G, Bech-Hansen N . Somatic segretation, recombination, asymmetrical distribution and complementation tests of cytoplasmically-inherited antibiotic-resistance mitochondrial markers in S. cerevisiae. Genetics. 1972; 72(1):1-15. PMC: 1212804. DOI: 10.1093/genetics/72.1.1. View

16.

Waxman M, Eaton N, Wilkie D . Effect of antibiotics on the transmission of mitochondrial factors in Saccharomyces cerevisiae. Mol Gen Genet. 1973; 127(4):277-84. DOI: 10.1007/BF00267098. View

17.

Thomas D, Wilkie D . Inhibition of mitochondrial synthesis in yeast by erythromycin: cytoplasmic and nuclear factors controlling resistance. Genet Res. 1968; 11(1):33-41. DOI: 10.1017/s0016672300011174. View