Molecular Typing of a BHV-4 (bovine Herpesvirus 4) Field Isolate

Overview

Journal Vet Res Commun

Publisher Springer

Specialty Veterinary Medicine

Date 2000 Oct 3

PMID 11014610

Citations 4

Authors

G DOnofrio

S Cavirani

C F Flammini

F SCATOZZA

Affiliations

Soon will be listed here.

Abstract

A new BHV-4 (bovine herpesvirus 4) isolated from a case of bovine interdigital dermatitis was characterized by PCR and restriction enzyme analysis. To determine whether the new isolate (PR/1) belonged to BHV-4, DNA from infected cells was specifically amplified by PCR. We used a set of primers spanning a large 2.571 kb conserved region of the BHV-4 genome, including the 3' end of ORF1 (homologous to the EBV BVRF1 gene), ORF2 (homologous to the EBV BXRF1 gene), ORF3 (TK gene) and ORF4 (gH gene) 5' end, respectively. The identity of the amplified product was confirmed by HindIII restriction enzyme digestion and Southern hybridization. No product was observed from the DNA of other bovine herpesviruses tested. The restriction patterns of the PR/ 1 genome compared to DN 599, MOVAR 33/63 and LVR BHV-4 reference strains showed two kinds of differences, either related or not related to the prDNA (polyrepetitive DNA). Taken together, these data show that PR/ 1 is a new BHV-4. We would consider that the present report provides a scheme of work for diagnosis and typing of BHV-4 isolates.

Citing Articles

Whole Genome Sequencing of a Canadian Bovine Gammaherpesvirus 4 Strain and the Possible Link between the Viral Infection and Respiratory and Reproductive Clinical Manifestations in Dairy Cattle.

Gagnon C, Traesel C, Music N, Laroche J, Tison N, Auger J Front Vet Sci. 2017; 4:92.

PMID: 28670580 PMC: 5472674. DOI: 10.3389/fvets.2017.00092.

Bovine Herpesvirus 4 in Parana State, Brazil: case report, viral isolation, and molecular identification.

Kruger E, Penha T, Stoffelo D, Roehe P, Ribeiro M, Soccol V Braz J Microbiol. 2015; 46(1):279-83.

PMID: 26221118 PMC: 4512082. DOI: 10.1590/S1517-838246120130949.

Isolation and characterization of bovine herpesvirus 4 (BoHV-4) from a cow affected by post partum metritis and cloning of the genome as a bacterial artificial chromosome.

Donofrio G, Franceschi V, Capocefalo A, Cavirani S, Sheldon I Reprod Biol Endocrinol. 2009; 7:83.

PMID: 19691825 PMC: 2734843. DOI: 10.1186/1477-7827-7-83.

Detection of bovine herpesvirus 4 (BoHV-4) DNA in the cell fraction of milk of dairy cattle with history of BoHV-4 infection.

DOnofrio G, Flammini C, SCATOZZA F, Cavirani S J Clin Microbiol. 2000; 38(12):4668-71.

PMID: 11101621 PMC: 87662. DOI: 10.1128/JCM.38.12.4668-4671.2000.

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