An Essential Octamer Motif in the Mb-1 (Igalpha) Promoter

The mb-1 (Igalpha) gene is B cell-specific and expressed throughout B cell maturation. In combination with B29 (Igbeta) and surface immunoglobulin mb-1 comprises the B cell receptor complex (BCR). The murine mb-1 promoter has been characterized to depend on the trans-acting transcription factors; Sp1, ets, lkaros, and EBF for full promoter activity. These trans-acting factors are also involved in the regulation of mb-1's closely related heterodimeric partner, B29. However, octamer transcription factors 1 and 2 (Oct-1 and Oct-2) are also necessary for full B29 promoter activity while they are not known to be required for mb-1 promoter activity. Here, we show that the octamer transcription factors bind a degenerate octamer consensus sequence within the mb-1 promoter. Like B29, the mb-1 octamer-binding, motif interacted with both ubiquitously expressed Oct-1 and the B cell-specific Oct-2 transcription factors. Furthermore, the interaction of Oct-1 and Oct-2 contributed to the regulation of the mb-1 promoter as site-directed mutations within the octamer motif substantially reduced its activity. These data confirm that octamer factor interactions and function contribute to the full transcriptional activity of the mb-1 promoter.