Derepression in Saccharomyces Cerevisiae Can Be Dissociated from Cellular Proliferation and Deoxyribonucleic Acid Synthesis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1975 Aug 1

PMID 1099068

Citations 6

Authors

H R Mahler

K Assimos

C C Lin

Affiliations

Soon will be listed here.

Abstract

A method has been developed that permits precise control of release from catabolite repression in Saccharomyces cerevisiae. It consists of transferring cells growing exponentially on 5% glucose to derepression medium at high cell density. Derepression then proceeds with reproducible kinetics and is complete within 6 to 7.5 h for various intra- and extramitochondrial markers, in the absence of any substantial increase in cellular dry weight or protein. Nuclear (and mitochondrial) deoxyribonucleic acid synthesis can be interrupted in certain thermosensitive (cdc) mutants at the nonpermissive temperature; a shift to this temperature before the onset of derepression has no effect on its outcome.

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Properties and possible functions of the adenylate cyclase in plasma membranes of Saccharomyces cerevisiae.

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Mitochondrial translation products during release from glucose repression in Saccharomyces cerevisiae.

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Effect of mitochondrial functions on synthesis of yeast cytochrome c.

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Molecular events during the release of delta-aminolevulinate dehydratase from catabolite repression.

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