Sindbis Virus Glycoprotein E1 is Divided into Two Discrete Domains at Amino Acid 129 by Disulfide Bridge Connections

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2000 Sep 12

PMID 10982379

Citations 2

Authors

B S Phinney

D T Brown

Affiliations

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Abstract

The E1 membrane glycoprotein of Sindbis virus contains structural and functional domains, which are conformationally dependent on the presence of intramolecular disulfide bridges (B. A. Abell and D. T. Brown, J. Virol. 67:5496-5501, 1993; R. P. Anthony, A. M. Paredes, and D. T. Brown, Virology 190:330-336, 1992). We have examined the disulfide bonds in E1 and have determined that the E1 membrane glycoprotein contains two separate sets of interconnecting disulfide linkages, which divide the protein into two domains at amino acid 129. These separate sets of disulfides may stabilize and define the structural and functional regions of the E1 protein.

Citing Articles

Structural differences observed in arboviruses of the alphavirus and flavivirus genera.

Hernandez R, Brown D, Paredes A Adv Virol. 2014; 2014:259382.

PMID: 25309597 PMC: 4182009. DOI: 10.1155/2014/259382.

Sindbis virus conformational changes induced by a neutralizing anti-E1 monoclonal antibody.

Hernandez R, Paredes A, Brown D J Virol. 2008; 82(12):5750-60.

PMID: 18417595 PMC: 2395122. DOI: 10.1128/JVI.02673-07.

References

Phinney B, Blackburn K, Brown D . The surface conformation of Sindbis virus glycoproteins E1 and E2 at neutral and low pH, as determined by mass spectrometry-based mapping. J Virol. 2000; 74(12):5667-78. PMC: 112055. DOI: 10.1128/jvi.74.12.5667-5678.2000. View

Kielian M, Klimjack M, Ghosh S, Duffus W . Mechanisms of mutations inhibiting fusion and infection by Semliki Forest virus. J Cell Biol. 1996; 134(4):863-72. PMC: 2120960. DOI: 10.1083/jcb.134.4.863. View

Burke D, Keegstra K . Purification and composition of the proteins from Sindbis virus grown in chick and BHK cells. J Virol. 1976; 20(3):676-86. PMC: 355045. DOI: 10.1128/JVI.20.3.676-686.1976. View

Miki T . Heterogeneity of Sindbis virus glycoprotein E1 and its modification by host cell transformation. J Gen Virol. 1984; 65 ( Pt 2):343-54. DOI: 10.1099/0022-1317-65-2-343. View

Kielian M, Keranen S, Kaariainen L, Helenius A . Membrane fusion mutants of Semliki Forest virus. J Cell Biol. 1984; 98(1):139-45. PMC: 2112989. DOI: 10.1083/jcb.98.1.139. View

ADAMS G, Rose J . Structural requirements of a membrane-spanning domain for protein anchoring and cell surface transport. Cell. 1985; 41(3):1007-15. DOI: 10.1016/s0092-8674(85)80081-7. View

Edwards J, Brown D . Sindbis virus-mediated cell fusion from without is a two-step event. J Gen Virol. 1986; 67 ( Pt 2):377-80. DOI: 10.1099/0022-1317-67-2-377. View

Marti T, Rosselet S, Titani K, Walsh K . Identification of disulfide-bridged substructures within human von Willebrand factor. Biochemistry. 1987; 26(25):8099-109. DOI: 10.1021/bi00399a013. View

Anthony R, Brown D . Protein-protein interactions in an alphavirus membrane. J Virol. 1991; 65(3):1187-94. PMC: 239885. DOI: 10.1128/JVI.65.3.1187-1194.1991. View

10.

Anthony R, Paredes A, Brown D . Disulfide bonds are essential for the stability of the Sindbis virus envelope. Virology. 1992; 190(1):330-6. DOI: 10.1016/0042-6822(92)91219-k. View

11.

Abell B, Brown D . Sindbis virus membrane fusion is mediated by reduction of glycoprotein disulfide bridges at the cell surface. J Virol. 1993; 67(9):5496-501. PMC: 237952. DOI: 10.1128/JVI.67.9.5496-5501.1993. View

12.

Mulvey M, Brown D . Formation and rearrangement of disulfide bonds during maturation of the Sindbis virus E1 glycoprotein. J Virol. 1994; 68(2):805-12. PMC: 236517. DOI: 10.1128/JVI.68.2.805-812.1994. View

13.

Bullough P, Hughson F, Skehel J, Wiley D . Structure of influenza haemagglutinin at the pH of membrane fusion. Nature. 1994; 371(6492):37-43. DOI: 10.1038/371037a0. View

14.

Blake C, Ghosh M, Harlos K, Avezoux A, Anthony C . The active site of methanol dehydrogenase contains a disulphide bridge between adjacent cysteine residues. Nat Struct Biol. 1994; 1(2):102-5. DOI: 10.1038/nsb0294-102. View

15.

STRAUSS Jr J, BURGE B, Darnell J . Carbohydrate content of the membrane protein of Sindbis virus. J Mol Biol. 1970; 47(3):437-48. DOI: 10.1016/0022-2836(70)90313-x. View