Cellular Location of Alpha-hemolysin in Staphylococcus Aureus

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1975 Aug 1

PMID 1097336

Authors

K M Nugent

G B Calandra

T S THEODORE

Affiliations

Soon will be listed here.

Abstract

Protoplast fractionation techniques were used to prepare subcellular fractions of three strains of Staphylococcus aureus for the analysis of alpha-hemolysin activity. More than 90% of the cellular hemolytic activity was localized in the periplasm, only a trace was detected in the cytoplasm, and none was found in either plasma membranes or mesosomes. This cellular portion constituted approximately 4 to 10% of the total hemolytic activity of the culture. The erythrocyte lysis spectrum and Sephadex G-100 filtration studies indicated that the cellular hemolysin closely resembles the extracellular form.

References

Burton K . A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956; 62(2):315-23. PMC: 1215910. DOI: 10.1042/bj0620315. View

Schwab J . An intracellular hemolysin of group A Streptococci. I. influence of sonic energy and pH on hemolytic potency. J Bacteriol. 1956; 71(1):94-9. PMC: 357741. DOI: 10.1128/jb.71.1.94-99.1956. View

BERNHEIMER A, SCHWARTZ L . Isolation and composition of staphylococcal alpha toxin. J Gen Microbiol. 1963; 30:455-68. DOI: 10.1099/00221287-30-3-455. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

COULTER J, Mukherjee T . Electron microscopic localization of alpha toxin within the staphylococcal cell by ferritin-labeled antibody. Infect Immun. 1971; 4(5):650-5. PMC: 416365. DOI: 10.1128/iai.4.5.650-655.1971. View

Andrews P . Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964; 91(2):222-33. PMC: 1202876. DOI: 10.1042/bj0910222. View

Nugent K, Huff E, Cole R, THEODORE T . Cellular location of degradative enzymes in Staphylococcus aureus. J Bacteriol. 1974; 120(3):1012-6. PMC: 245878. DOI: 10.1128/jb.120.3.1012-1016.1974. View

McNiven A, Arbuthnott J . Cell-associated alpha-toxin from Staphylococcus aureus. J Med Microbiol. 1972; 5(1):123-7. DOI: 10.1099/00222615-5-1-123. View

COULTER J . Production, purification, and composition of staphylococcal alpha toxin. J Bacteriol. 1966; 92(6):1655-62. PMC: 316245. DOI: 10.1128/jb.92.6.1655-1662.1966. View

10.

Arbuthnott J, FREER J, BERNHEIMER A . Physical states of staphylococcal alpha-toxin. J Bacteriol. 1967; 94(4):1170-7. PMC: 276791. DOI: 10.1128/jb.94.4.1170-1177.1967. View

11.

THEODORE T, Popkin T, Cole R . The separation and isolation of plasma membranes and mesosomal vesicles from Staphylococcus aureus. Prep Biochem. 1971; 1(3):233-48. DOI: 10.1080/00327487108081942. View

12.

Duncan J, Cho G . Production of staphylococcal alpha toxin. I. Relationship between cell growth and toxin formation. Infect Immun. 1971; 4(4):456-61. PMC: 416331. DOI: 10.1128/iai.4.4.456-461.1971. View

13.

Forlani L, BERNHEIMER A, CHIANCONE E . Ultracentrifugal analysis of staphylococcal alpha toxin. J Bacteriol. 1971; 106(1):138-42. PMC: 248653. DOI: 10.1128/jb.106.1.138-142.1971. View

14.

Weber K, Osborn M . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969; 244(16):4406-12. View