Isolation and Characterization of Vibrio Parahaemolyticus from Cape Cod Soft-shell Clams (Mya Arenaria)

Overview

Journal Appl Microbiol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1975 May 1

PMID 1096817

Citations 6

Authors

P M Earle

F D Crisley

Affiliations

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Abstract

Vibrio parahaemolyticus was isolated from soft-shell clams (Mya arenaria) taken from 10 different clamming areas on Cape Cod, Mass., during July and August 1972. Direct plating on thiosulfate-citrate-bile salts-sucrose agar was found to be superior to either direct plating on Vanderzant modified salt starch agar or enrichment with Trypticase soy broth containing 7% salt for isolation from clam samples. Morphological and biochemical characteristics of 33 isolates from 30 samples generally conform to those described for this organism in the literature, except for the production of acid from sucrose, lactose, and sorbitol. Six of the isolates were hemolytic on human blood agar plates, whereas all showed a negative Kanagawa phenomenon. Twenty of the 33 isolates reacted with pooled antisera to the K antigen; 15 of these reacted with 9 different specific K antisera, leaving 5 untypable. Ten of these 15 reacted with 4 different O antisera.

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Molecular, serological, and virulence characteristics of Vibrio parahaemolyticus isolated from environmental, food, and clinical sources in North America and Asia.

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Incidence of Vibrio parahaemolyticus in U.S. coastal waters and oysters.

DePAOLA A, Hopkins L, Peeler J, Wentz B, McPHEARSON R Appl Environ Microbiol. 1990; 56(8):2299-302.

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