Plant Genome Complexity May Be a Factor Limiting in Situ the Transfer of Transgenic Plant Genes to the Phytopathogen Ralstonia Solanacearum

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2000 Aug 31

PMID 10966449

Citations 5

Authors

F Bertolla

R Pepin

E Paget

A Simkin

X Nesme

P Simonet

Affiliations

Soon will be listed here.

Abstract

The development of natural competence by bacteria in situ is considered one of the main factors limiting transformation-mediated gene exchanges in the environment. Ralstonia solanacearum is a plant pathogen that is also a naturally transformable bacterium that can develop the competence state during infection of its host. We have attempted to determine whether this bacterium could become the recipient of plant genes. We initially demonstrated that plant DNA was released close to the infecting bacteria. We constructed and tested various combinations of transgenic plants and recipient bacteria to show that the effectiveness of such transfers was directly related to the ratio of the complexity of the plant genome to the number of copies of the transgene.

Citing Articles

Next-generation marker-free transplastomic plants: engineering the chloroplast genome without integration of marker genes in Solanum tuberosum (potato).

Occhialini A, Reed A, Harbison S, Sichterman M, Baumann A, Pfotenhauer A Plant Cell Rep. 2024; 43(12):290.

PMID: 39578272 DOI: 10.1007/s00299-024-03375-9.

Could DNA uptake be a side effect of bacterial adhesion and twitching motility?.

Bakkali M Arch Microbiol. 2013; 195(4):279-89.

PMID: 23381940 PMC: 3597990. DOI: 10.1007/s00203-013-0870-1.

Degradation and transformability of DNA from transgenic leaves.

Ceccherini M, Pote J, Kay E, Tran Van V, Marechal J, Pietramellara G Appl Environ Microbiol. 2003; 69(1):673-8.

PMID: 12514059 PMC: 152424. DOI: 10.1128/AEM.69.1.673-678.2003.

Plant-specific promoter sequences carry elements that are recognised by the eubacterial transcription machinery.

Jacob D, Lewin A, Meister B, Appel B Transgenic Res. 2002; 11(3):291-303.

PMID: 12113461 DOI: 10.1023/a:1015620016472.

In situ transfer of antibiotic resistance genes from transgenic (transplastomic) tobacco plants to bacteria.

Kay E, Vogel T, Bertolla F, Nalin R, Simonet P Appl Environ Microbiol. 2002; 68(7):3345-51.

PMID: 12089013 PMC: 126776. DOI: 10.1128/AEM.68.7.3345-3351.2002.

References

Brau B, Pilz U, Piepersberg W . Genes for gentamicin-(3)-N-acetyltransferases III and IV: I. Nucleotide sequence of the AAC(3)-IV gene and possible involvement of an IS140 element in its expression. Mol Gen Genet. 1984; 193(1):179-87. DOI: 10.1007/BF00327434. View

Reynolds E . The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963; 17:208-12. PMC: 2106263. DOI: 10.1083/jcb.17.1.208. View

Ubben D, Schmitt R . A transposable promoter and transposable promoter probes derived from Tn1721. Gene. 1987; 53(1):127-34. DOI: 10.1016/0378-1119(87)90100-4. View

Roberts D, Denny T, Schell M . Cloning of the egl gene of Pseudomonas solanacearum and analysis of its role in phytopathogenicity. J Bacteriol. 1988; 170(4):1445-51. PMC: 210987. DOI: 10.1128/jb.170.4.1445-1451.1988. View

Schell M, Roberts D, Denny T . Analysis of the Pseudomonas solanacearum polygalacturonase encoded by pglA and its involvement in phytopathogenicity. J Bacteriol. 1988; 170(10):4501-8. PMC: 211482. DOI: 10.1128/jb.170.10.4501-4508.1988. View

Mersereau M, Pazour G, Das A . Efficient transformation of Agrobacterium tumefaciens by electroporation. Gene. 1990; 90(1):149-51. DOI: 10.1016/0378-1119(90)90452-w. View

Spok A, Stubenrauch G, Schorgendorfer K, Schwab H . Molecular cloning and sequencing of a pectinesterase gene from Pseudomonas solanacearum. J Gen Microbiol. 1991; 137(1):131-40. DOI: 10.1099/00221287-137-1-131. View

Khasanov F, Zvingila D, Zainullin A, Prozorov A, Bashkirov V . Homologous recombination between plasmid and chromosomal DNA in Bacillus subtilis requires approximately 70 bp of homology. Mol Gen Genet. 1992; 234(3):494-7. DOI: 10.1007/BF00538711. View

Smith M, Feng D, Doolittle R . Evolution by acquisition: the case for horizontal gene transfers. Trends Biochem Sci. 1992; 17(12):489-93. DOI: 10.1016/0968-0004(92)90335-7. View

10.

Bickle T, Kruger D . Biology of DNA restriction. Microbiol Rev. 1993; 57(2):434-50. PMC: 372918. DOI: 10.1128/mr.57.2.434-450.1993. View

11.

Arlat M, Van Gijsegem F, Huet J, Pernollet J, Boucher C . PopA1, a protein which induces a hypersensitivity-like response on specific Petunia genotypes, is secreted via the Hrp pathway of Pseudomonas solanacearum. EMBO J. 1994; 13(3):543-53. PMC: 394843. DOI: 10.1002/j.1460-2075.1994.tb06292.x. View

12.

Hajdukiewicz P, Svab Z, Maliga P . The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol Biol. 1994; 25(6):989-94. DOI: 10.1007/BF00014672. View

13.

Lorenz M, Wackernagel W . Bacterial gene transfer by natural genetic transformation in the environment. Microbiol Rev. 1994; 58(3):563-602. PMC: 372978. DOI: 10.1128/mr.58.3.563-602.1994. View

14.

Matic I, Rayssiguier C, Radman M . Interspecies gene exchange in bacteria: the role of SOS and mismatch repair systems in evolution of species. Cell. 1995; 80(3):507-15. DOI: 10.1016/0092-8674(95)90501-4. View

15.

Normand P, Orso S, Cournoyer B, Jeannin P, Chapelon C, Dawson J . Molecular phylogeny of the genus Frankia and related genera and emendation of the family Frankiaceae. Int J Syst Bacteriol. 1996; 46(1):1-9. DOI: 10.1099/00207713-46-1-1. View

16.

Bertolla F, Van Gijsegem F, Nesme X, Simonet P . Conditions for natural transformation of Ralstonia solanacearum. Appl Environ Microbiol. 1997; 63(12):4965-8. PMC: 168825. DOI: 10.1128/aem.63.12.4965-4968.1997. View

17.

Gebhard F, Smalla K . Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA. Appl Environ Microbiol. 1998; 64(4):1550-4. PMC: 106187. DOI: 10.1128/AEM.64.4.1550-1554.1998. View

18.

de Vries J, Wackernagel W . Detection of nptII (kanamycin resistance) genes in genomes of transgenic plants by marker-rescue transformation. Mol Gen Genet. 1998; 257(6):606-13. DOI: 10.1007/s004380050688. View

19.

Schluter K, Futterer J, Potrykus I . "Horizontal" gene transfer from a transgenic potato line to a bacterial pathogen (Erwinia chrysanthemi) occurs--if at all--at an extremely low frequency. Biotechnology (N Y). 1995; 13(10):1094-8. DOI: 10.1038/nbt1095-1094. View

20.

Bertolla F, Simonet P . Horizontal gene transfers in the environment: natural transformation as a putative process for gene transfers between transgenic plants and microorganisms. Res Microbiol. 1999; 150(6):375-84. DOI: 10.1016/s0923-2508(99)80072-2. View