Endonucleolytic Incision of X-irradiated Deoxyribonucleic Acid by Extracts of Escherichia Coli

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1975 Jun 1

PMID 1094450

Citations 13

Authors

G F Strniste

S S Wallace

Affiliations

Soon will be listed here.

Abstract

An enconuclease activity that reacts with x-irradiated DNA is present in extracts of E. coli. By using centrifugal methods to monitor the conversion of the supercoiled, circular double-stranded DNA for phage phi-x-174 (replicative form) or PM2 to the relaxed circular form it was possible to quantitate the rate of radiation induced endonuclease-sensitive sites in the DNA. For every single-strand break induced by x-rays under aerobic irradiation conditions, there is approximately one induced site sensitive to this endonuclease activity. Under irradiation conditions (addition OF Potassium iodide) that dramatically reduce rates of single-strand breaks and "alkalilabile" lesions, the number of endonuclease-sensitive sites relative to single-strand breaks increase approximatley 4-fold. This nuclease is present in several strains of E. coli B and K12, including mutants deficient in DNA polymerase I, recombination gene products (rec mutants), ultraviolet light incision enzyme (uvr A mutant), and endonuclease II. It is suggested that this endonuclease may be involved in an excision repair process for damages incurred in DNA by ionizing radiation.

Citing Articles

Hide and seek: How do DNA glycosylases locate oxidatively damaged DNA bases amidst a sea of undamaged bases?.

Lee A, Wallace S Free Radic Biol Med. 2016; 107:170-178.

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DNA Processing Proteins Involved in the UV-Induced Stress Response of Sulfolobales.

van Wolferen M, Ma X, Albers S J Bacteriol. 2015; 197(18):2941-51.

PMID: 26148716 PMC: 4542170. DOI: 10.1128/JB.00344-15.

Base excision repair: a critical player in many games.

Wallace S DNA Repair (Amst). 2014; 19:14-26.

PMID: 24780558 PMC: 4100245. DOI: 10.1016/j.dnarep.2014.03.030.

Insights into the glycosylase search for damage from single-molecule fluorescence microscopy.

Lee A, Warshaw D, Wallace S DNA Repair (Amst). 2014; 20:23-31.

PMID: 24560296 PMC: 4112009. DOI: 10.1016/j.dnarep.2014.01.007.

DNA glycosylases search for and remove oxidized DNA bases.

Wallace S Environ Mol Mutagen. 2013; 54(9):691-704.

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