Estrogen Induction of Ovalbumin MRNA: Evidence for Transcription Control

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 1975 Apr 30

PMID 1094273

Citations 5

Authors

A R Means

S Woo

S E Harris

B W OMalley

Affiliations

Soon will be listed here.

Abstract

Estrogen induces the synthesis and accumulation of the specific messenger RNA for the egg white protein ovalbumin. The messenger RNA has been purified to apparent homogeneity on a preparative scale and utilized to synthesize a radioactive complementary DNA copy. This complementary DNA probe was first used to reveal that although ovalbumin constitutes 60% of the total protein of chick oviduct the gene which codes for the ovalbumin nRNA is represented only once in each haploid genome: The induction of genetranscription and subsequent accumulation of ovalbumin mRNA during estrogen-mediated tissue differentiation was also investigated. Ovalbumin mRNA sequences were quantifiedusing the complementary DNA probe and by an in vitro heterologous translation system. Similar experiments were performed using chicks which were withdrawn from hormone treatment and then given a single injection of estrogen. The data suggest pure transcriptional control for the mechanism by which estrogen regulates the synthesis of the tissuespecific protein ovalbumin. Finally, several in vitro translation systems are comparedwith respect to their usefullness to assess the effects of hormones on mRNA production. It is concluded that the protein synthesis system derived from wheat germ offers thegreatest advantages for initial studies.

Citing Articles

Evolutionary transcriptomics implicates in the origins of implantation and regulation of gestation length.

Marinic M, Mika K, Chigurupati S, Lynch V Elife. 2021; 10.

PMID: 33522483 PMC: 7943190. DOI: 10.7554/eLife.61257.

The regulation of protein synthesis in heart muscle under normal conditions and in the adriamycin-cardiomyopathy.

Zahringer J Klin Wochenschr. 1981; 59(23):1273-87.

PMID: 6171675 DOI: 10.1007/BF01711177.

Characterization of uterine heterogeneous nuclear ribonucleic acid and the effect of oestradiol-17 beta on its synthesis.

Aziz S, Knowler J Biochem J. 1978; 172(3):587-93.

PMID: 687360 PMC: 1185734. DOI: 10.1042/bj1720587.

Steroid hormone receptors in the regulation of differentiation. A review.

McCarty Jr K, McCarty Sr K Am J Pathol. 1977; 86(3):705-44.

PMID: 190892 PMC: 2032117.

Synthesis of very low density lipoproteins in the cockerel. Effects of estrogen.

Chan L, Jackson R, OMalley B, Means A J Clin Invest. 1976; 58(2):368-79.

PMID: 182719 PMC: 333192. DOI: 10.1172/JCI108481.

References

Sullivan D, Palacios R, Stavnezer J, Taylor J, Faras A, Kiely M . Synthesis of a deoxyribonucleic acid sequence complementary to ovalbumin messenger ribonucleic acid and quantification of ovalbumin genes. J Biol Chem. 1973; 248(21):7530-9. View

Means A, OMalley B . Mechanism of estrogen action: early transcriptional and translational events. Metabolism. 1972; 21(4):357-70. DOI: 10.1016/0026-0495(72)90081-9. View

Mathews M, Korner A . Mammalian cell-free protein synthesis directed by viral ribonucleic acid. Eur J Biochem. 1970; 17(2):328-38. DOI: 10.1111/j.1432-1033.1970.tb01170.x. View

Jacob F, MONOD J . Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961; 3:318-56. DOI: 10.1016/s0022-2836(61)80072-7. View

Brawerman G, MENDECKI J, Lee S . A procedure for the isolation of mammalian messenger ribonucleic acid. Biochemistry. 1972; 11(4):637-41. DOI: 10.1021/bi00754a027. View

Means A, OMalley B . Assessment of sex steroid action in vitro. Acta Endocrinol Suppl (Copenh). 1971; 153:318-36. DOI: 10.1530/acta.0.068s318. View

OMalley B, Rosenfeld G, Comstock J, Means A . Steroid hormone induction of a specific translatable messenger RNA. Nat New Biol. 1972; 240(97):45-8. DOI: 10.1038/newbio240045a0. View

Comstock J, OMalley B, Means A . Stimulation of cell-free polypeptide synthesis by a protein fraction extracted from chick oviduct polyribosomes. Biochemistry. 1972; 11(4):646-52. DOI: 10.1021/bi00754a029. View

Palacios R, Sullivan D, Summers N, Kiely M, Schimke R . Purification of ovalbumin messenger ribonucleic acid by specific immunoadsorption of ovalbumin-synthesizing polysomes and millipore partition of ribonucleic acid. J Biol Chem. 1973; 248(2):540-8. View

10.

Rhoads R, McKnight G, Schimke R . Synthesis of ovalbumin in a rabbit reticulocyte cell-free system programmed with hen oviduct ribonucleic acid. J Biol Chem. 1971; 246(23):7407-10. View

11.

Kacian D, Watson K, Burny A, Spiegelman S . Purification of the DNA polymerase of avian myeloblastosis virus. Biochim Biophys Acta. 1971; 246(3):365-83. DOI: 10.1016/0005-2787(71)90773-8. View

12.

Comstock J, Rosenfeld G, OMalley B, Means A . Estrogen-induced changes in translation, and specific messenger RNA levels during oviduct differentiation. Proc Natl Acad Sci U S A. 1972; 69(9):2377-80. PMC: 426944. DOI: 10.1073/pnas.69.9.2377. View

13.

Roberts B, Paterson B . Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973; 70(8):2330-4. PMC: 433729. DOI: 10.1073/pnas.70.8.2330. View

14.

Harris S, Means A, MITCHELL W, OMalley B . Synthesis of (3H)DNA complementary to ovalbumin messenger RNA: evidence for limited copies of the ovalbumin gene in chick oviduct. Proc Natl Acad Sci U S A. 1973; 70(12):3776-80. PMC: 427326. DOI: 10.1073/pnas.70.12.3776. View

15.

Means A, Comstock J, Rosenfeld G, OMalley B . Ovalbumin messenger RNA of chick oviduct: partial characterization, estrogen dependence, and translation in vitro. Proc Natl Acad Sci U S A. 1972; 69(5):1146-50. PMC: 426650. DOI: 10.1073/pnas.69.5.1146. View

16.

OMalley B, McGuire W, Kohler P, Korenman S . Studies on the mechanism of steroid hormone regulation of synthesis of specific proteins. Recent Prog Horm Res. 1969; 25:105-60. DOI: 10.1016/b978-0-12-571125-8.50006-5. View

17.

Chan L, Means A, OMalley B . Rates of induction of specific translatable messenger RNAs for ovalbumin and avidin by steroid hormones. Proc Natl Acad Sci U S A. 1973; 70(6):1870-4. PMC: 433615. DOI: 10.1073/pnas.70.6.1870. View

18.

OMalley B, Means A . Female steroid hormones and target cell nuclei. Science. 1974; 183(4125):610-20. DOI: 10.1126/science.183.4125.610. View

19.

Rosenfeld G, Comstock J, Means A, OMalley B . Estrogen-induced synthesis of ovalbumin messenger RNA and its translation in a cell-free system. Biochem Biophys Res Commun. 1972; 46(4):1695-703. DOI: 10.1016/0006-291x(72)90805-4. View

20.

Marcus A, Weeks D, Leis J, KELLER E . Protein chain initiation by methionyl-tRNA in wheat embryo. Proc Natl Acad Sci U S A. 1970; 67(4):1681-7. PMC: 283412. DOI: 10.1073/pnas.67.4.1681. View