Purification and Properties of a Bacteriophage T5-modified Form of Escherichia Coli RNA Polymerase

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1975 Apr 1

PMID 1090747

Citations 10

Authors

C Szabo

R W Moyer

Affiliations

Soon will be listed here.

Abstract

A modified form of Escherichia coli RNA polymerase that contains one of the three T5-specific polypeptides known to interact with the host enzyme was purified from bacteriophage T5-infected cells. The properties of this T5-modified enzyme appeared identical to those of the RNA polymerase derived from uninfected non-colicinogenic cells and to a fully active enzyme isolated from T5-infected ColIb+ cells after the limited in vivo transcription of T5 genes allowed by the plasmid had ceased.

Citing Articles

Novel RNA Polymerase Binding Protein Encoded by Bacteriophage T5.

Klimuk E, Mekler V, Lavysh D, Serebryakova M, Akulenko N, Severinov K Viruses. 2020; 12(8).

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Pre-early functions of bacteriophage T5 and its relatives.

Davison J Bacteriophage. 2016; 5(4):e1086500.

PMID: 26904381 PMC: 4743489. DOI: 10.1080/21597081.2015.1086500.

Modification of RNA polymerase from Escherichia coli by pre-early gene products of bacteriophage T5.

McCorquodale D, Chen C, Joseph M, Woychik R J Virol. 1981; 40(3):958-62.

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Inhibition of bacteriophage replication by extrachromosomal genetic elements.

Duckworth D, Glenn J, McCorquodale D Microbiol Rev. 1981; 45(1):52-71.

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Transcription regulatory elements in the late region of bacteriophage T5 DNA.

Brunel F, Thi V, Pilaete M, Davison J Nucleic Acids Res. 1983; 11(21):7649-58.

PMID: 6316267 PMC: 326509. DOI: 10.1093/nar/11.21.7649.

References

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