Active Zones on Motor Nerve Terminals Contain Alpha 3beta 1 Integrin

Overview

Journal J Neurosci

Specialty Neurology

Date 2000 Jun 24

PMID 10864949

Citations 17

Authors

M W Cohen

B G Hoffstrom

D W DeSimone

Affiliations

Soon will be listed here.

Abstract

Active zones are the sites along nerve terminals where synaptic vesicles dock and undergo calcium-dependent exocytosis during synaptic transmission. Here we show, by immunofluorescent staining with antibodies generated against Xenopus laevis integrins, that alpha3beta1 integrin is concentrated at the active zones of Xenopus motor nerve terminals. Because integrins can link extracellular matrix molecules to cytoskeletal elements and participate in the formation of signaling complexes, the localization of integrin at active zones suggests that it may play a role in the adhesion of the nerve terminals to the synaptic basal lamina, in the formation and maintenance of active zones, and in some of the events associated with calcium-dependent exocytosis of neurotransmitter. Our findings also indicate that the integrin composition of the terminal Schwann cells differs from that of the motor nerve terminals, and this may account at least in part for differences in their adhesiveness to the synaptic basal lamina.

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References

Gawantka V, Joos T, Hausen P . A beta 1-integrin associated alpha-chain is differentially expressed during Xenopus embryogenesis. Mech Dev. 1994; 47(3):199-211. DOI: 10.1016/0925-4773(94)90039-6. View

Patton B, Miner J, Chiu A, Sanes J . Distribution and function of laminins in the neuromuscular system of developing, adult, and mutant mice. J Cell Biol. 1998; 139(6):1507-21. PMC: 2132624. DOI: 10.1083/jcb.139.6.1507. View

Pinkstaff J, Lynch G, Gall C . Localization and seizure-regulation of integrin beta 1 mRNA in adult rat brain. Brain Res Mol Brain Res. 1998; 55(2):265-76. DOI: 10.1016/s0169-328x(98)00007-2. View

Trachtenberg J, Thompson W . Nerve terminal withdrawal from rat neuromuscular junctions induced by neuregulin and Schwann cells. J Neurosci. 1997; 17(16):6243-55. PMC: 6568340. View

Bozyczko D, Decker C, Muschler J, Horwitz A . Integrin on developing and adult skeletal muscle. Exp Cell Res. 1989; 183(1):72-91. DOI: 10.1016/0014-4827(89)90419-9. View

Dodd J, Jessell T . Axon guidance and the patterning of neuronal projections in vertebrates. Science. 1988; 242(4879):692-9. DOI: 10.1126/science.3055291. View

Martin P, Kaufman S, Kramer R, Sanes J . Synaptic integrins in developing, adult, and mutant muscle: selective association of alpha1, alpha7A, and alpha7B integrins with the neuromuscular junction. Dev Biol. 1996; 174(1):125-39. DOI: 10.1006/dbio.1996.0057. View

Gawantka V, Ellinger-Ziegelbauer H, Hausen P . Beta 1-integrin is a maternal protein that is inserted into all newly formed plasma membranes during early Xenopus embryogenesis. Development. 1992; 115(2):595-605. DOI: 10.1242/dev.115.2.595. View

Robitaille R, Adler E, Charlton M . Strategic location of calcium channels at transmitter release sites of frog neuromuscular synapses. Neuron. 1990; 5(6):773-9. DOI: 10.1016/0896-6273(90)90336-e. View

10.

Anderson M, Shi Z, Zackson S . Proteolytic disruption of laminin-integrin complexes on muscle cells during synapse formation. Mol Cell Biol. 1996; 16(9):4972-84. PMC: 231499. DOI: 10.1128/MCB.16.9.4972. View

11.

Nishimura S, Boylen K, Einheber S, Milner T, Ramos D, Pytela R . Synaptic and glial localization of the integrin alphavbeta8 in mouse and rat brain. Brain Res. 1998; 791(1-2):271-82. DOI: 10.1016/s0006-8993(98)00118-8. View

12.

Dreyer F, Peper K, Akert K, Sandri C, Moor H . Ultrastructure of the "active zone" in the frog neuromuscular junction. Brain Res. 1973; 62(2):373-80. DOI: 10.1016/0006-8993(73)90699-9. View

13.

Betz W, Sakmann B . Effects of proteolytic enzymes on function and structure of frog neuromuscular junctions. J Physiol. 1973; 230(3):673-88. PMC: 1350622. DOI: 10.1113/jphysiol.1973.sp010211. View

14.

Belkin A, Zhidkova N, Balzac F, Altruda F, Tomatis D, Maier A . Beta 1D integrin displaces the beta 1A isoform in striated muscles: localization at junctional structures and signaling potential in nonmuscle cells. J Cell Biol. 1996; 132(1-2):211-26. PMC: 2120711. DOI: 10.1083/jcb.132.1.211. View

15.

Robitaille R . Modulation of synaptic efficacy and synaptic depression by glial cells at the frog neuromuscular junction. Neuron. 1998; 21(4):847-55. DOI: 10.1016/s0896-6273(00)80600-5. View

16.

Einheber S, Schnapp L, Salzer J, Cappiello Z, Milner T . Regional and ultrastructural distribution of the alpha 8 integrin subunit in developing and adult rat brain suggests a role in synaptic function. J Comp Neurol. 1996; 370(1):105-34. DOI: 10.1002/(SICI)1096-9861(19960617)370:1<105::AID-CNE10>3.0.CO;2-R. View

17.

Sanes J, Lichtman J . Development of the vertebrate neuromuscular junction. Annu Rev Neurosci. 1999; 22:389-442. DOI: 10.1146/annurev.neuro.22.1.389. View

18.

Son Y, Trachtenberg J, Thompson W . Schwann cells induce and guide sprouting and reinnervation of neuromuscular junctions. Trends Neurosci. 1996; 19(7):280-5. DOI: 10.1016/S0166-2236(96)10032-1. View

19.

Wayner E, Carter W . Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits. J Cell Biol. 1987; 105(4):1873-84. PMC: 2114635. DOI: 10.1083/jcb.105.4.1873. View

20.

Sanes J, Marshall L, McMahan U . Reinnervation of muscle fiber basal lamina after removal of myofibers. Differentiation of regenerating axons at original synaptic sites. J Cell Biol. 1978; 78(1):176-98. PMC: 2110176. DOI: 10.1083/jcb.78.1.176. View