T-type Alpha 1H Ca2+ Channels Are Involved in Ca2+ Signaling During Terminal Differentiation (fusion) of Human Myoblasts

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2000 Jun 22

PMID 10861024

Citations 74

Authors

P Bijlenga

J H Liu

E Espinos

C A Haenggeli

J Fischer-Lougheed

C R Bader

L Bernheim

Affiliations

Soon will be listed here.

Abstract

Mechanisms underlying Ca(2+) signaling during human myoblast terminal differentiation were studied using cell cultures. We found that T-type Ca(2+) channels (T-channels) are expressed in myoblasts just before fusion. Their inhibition by amiloride or Ni(2+) suppresses fusion and prevents an intracellular Ca(2+) concentration increase normally observed at the onset of fusion. The use of antisense oligonucleotides indicates that the functional T-channels are formed by alpha1H subunits. At hyperpolarized potentials, these channels allow a window current sufficient to increase [Ca(2+)](i). As hyperpolarization is a prerequisite to myoblast fusion, we conclude that the Ca(2+) signal required for fusion is produced when the resting potential enters the T-channel window. A similar mechanism could operate in other cell types of which differentiation implicates membrane hyperpolarization.

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