Changes in Tissue-plasminogen Activator MRNA Expression Following Cortical Ablation in the Rat Brain

Overview

Journal J Mol Neurosci

Specialties Molecular Biology
Neurology

Date 2000 Jun 15

PMID 10854036

Authors

E Kohmura

T Yuguchi

T Sakaki

M Nonaka

T Fujinaka

T Hayakawa

T Yoshimine

Affiliations

Soon will be listed here.

Abstract

Tissue plasminogen activator (tPA) has been used to treat acute thrombotic lesions. Roles other than the activation of fibrinolytic pathways have been suggested for tPA in the mature brain. We used the in situ hybridization technique to investigate the changes in tPA mRNA expression within the brain after cortical ablation. We found that expression of tPA mRNA started to increase diffusely in the cortex ipsilateral to the injury 6 h after ablation. This increase had become prominent 24 h after ablation. On d 5, the expression of tPA mRNA had returned to that of the control animals except for the area near the injury. We also found that administration of MK-801 before injury suppressed the increase of tPA mRNA in the ipsilateral cortex. These results suggest that the increase in tPA mRNA is likely to be mediated via activation of NMDA receptors.

References

Tsirka S . Clinical implications of the involvement of tPA in neuronal cell death. J Mol Med (Berl). 1997; 75(5):341-7. DOI: 10.1007/s001090050119. View

Kohmura E, Yuguchi T, Yamada K, Sakaguchi T, Wanaka A, Hayakawa T . Expression of c-fos mRNA after cortical ablation in rat brain is modulated by basic fibroblast growth factor (bFGF) and the NMDA receptor is involved in c-fos expression. Brain Res Mol Brain Res. 1995; 28(1):117-21. DOI: 10.1016/0169-328x(94)00198-n. View

Wang Y, Tsirka S, Strickland S, Stieg P, Soriano S, Lipton S . Tissue plasminogen activator (tPA) increases neuronal damage after focal cerebral ischemia in wild-type and tPA-deficient mice. Nat Med. 1998; 4(2):228-31. DOI: 10.1038/nm0298-228. View

Ware J, Dibenedetto A, Pittman R . Localization of tissue plasminogen activator mRNA in adult rat brain. Brain Res Bull. 1995; 37(3):275-81. DOI: 10.1016/0361-9230(95)00008-3. View

Moonen G, Selak I . Plasminogen activator-plasmin system and neuronal migration. Nature. 1982; 298(5876):753-5. DOI: 10.1038/298753a0. View

Yuguchi T, Kohmura E, Yamada K, Wanaka A, Otsuki H, Sakaguchi T . Messenger RNA and protein expression of basic fibroblast growth factor receptor after cortical ablation. Brain Res Mol Brain Res. 1994; 25(1-2):50-6. DOI: 10.1016/0169-328x(94)90277-1. View

Gualandris A, Jones T, Strickland S, Tsirka S . Membrane depolarization induces calcium-dependent secretion of tissue plasminogen activator. J Neurosci. 1996; 16(7):2220-5. PMC: 6578519. View

Krystosek A, Seeds N . Plasminogen activator release at the neuronal growth cone. Science. 1981; 213(4515):1532-4. DOI: 10.1126/science.7197054. View

Sharp J, Sagar S, Hisanaga K, Jasper P, Sharp F . The NMDA receptor mediates cortical induction of fos and fos-related antigens following cortical injury. Exp Neurol. 1990; 109(3):323-32. DOI: 10.1016/s0014-4886(05)80023-8. View

10.

Seeds N, Williams B, Bickford P . Tissue plasminogen activator induction in Purkinje neurons after cerebellar motor learning. Science. 1995; 270(5244):1992-4. DOI: 10.1126/science.270.5244.1992. View

11.

Yuguchi T, Kohmura E, Yamada K, Otsuki H, Sakaki T, Yamashita T . Expression of tPA mRNA in the facial nucleus following facial nerve transection in the rat. Neuroreport. 1997; 8(2):419-22. DOI: 10.1097/00001756-199701200-00008. View

12.

Moonen G, Selak I, Lefebvre P, Rogister B, Vassalli J, Belin D . Plasminogen activator is a mitogen for astrocytes in developing cerebellum. Brain Res. 1985; 352(1):41-8. DOI: 10.1016/0165-3806(85)90085-9. View

13.

Reddington M, Haas C, Kreutzberg G . The plasminogen activator system in neurons and glia during motoneuron regeneration. Neuropathol Appl Neurobiol. 1994; 20(2):188-90. View

14.

Scarisbrick I, Towner M, Isackson P . Nervous system-specific expression of a novel serine protease: regulation in the adult rat spinal cord by excitotoxic injury. J Neurosci. 1997; 17(21):8156-68. PMC: 6573769. View

15.

Kalderon N . Schwann cell proliferation and localized proteolysis: expression of plasminogen-activator activity predominates in the proliferating cell populations. Proc Natl Acad Sci U S A. 1984; 81(22):7216-20. PMC: 392109. DOI: 10.1073/pnas.81.22.7216. View

16.

Kariko K, Harris V, Rangel Y, Duvall M, Welsh F . Effect of cortical spreading depression on the levels of mRNA coding for putative neuroprotective proteins in rat brain. J Cereb Blood Flow Metab. 1998; 18(12):1308-15. DOI: 10.1097/00004647-199812000-00005. View

17.

Pennica D, Holmes W, Kohr W, Harkins R, Vehar G, Ward C . Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli. Nature. 1983; 301(5897):214-21. DOI: 10.1038/301214a0. View

18.

Rickles R, Darrow A, Strickland S . Molecular cloning of complementary DNA to mouse tissue plasminogen activator mRNA and its expression during F9 teratocarcinoma cell differentiation. J Biol Chem. 1988; 263(3):1563-9. View

19.

Hekman C, Loskutoff D . Kinetic analysis of the interactions between plasminogen activator inhibitor 1 and both urokinase and tissue plasminogen activator. Arch Biochem Biophys. 1988; 262(1):199-210. DOI: 10.1016/0003-9861(88)90182-8. View

20.

Tsirka S, Rogove A, Bugge T, Degen J, Strickland S . An extracellular proteolytic cascade promotes neuronal degeneration in the mouse hippocampus. J Neurosci. 1997; 17(2):543-52. PMC: 6573220. View