Expression of Murine Adenosine Deaminase (ADA) in Transgenic Maize

Overview

Journal Transgenic Res

Specialty Molecular Biology

Date 2000 Jun 15

PMID 10853264

Citations 2

Authors

J F Petolino

S Young

N Hopkins

K Sukhapinda

A Woosley

C Hayes

L Pelcher

Affiliations

Soon will be listed here.

Abstract

A murine adenosine deaminase (ADA) gene, driven by the maize ubi-1 promoter and intron region, was transformed into embryogenic maize callus, along with a bar and gusA gene-containing plasmid, using microparticle bombardment. Selection in the presence of either the herbicide Basta or the adenosine analogue 2'-deoxyadenosine resulted in transgenic cultures that expressed GUS and accumulated a 41-kD protein that immunoprecipated with an ADA-specific polyclonal antibody. ADA enzyme activity was observed in extracts from transgenic callus as well as regenerated plants and progeny. Culltures expressing ADA grew in the presence of 200 mg/l 2'-deoxyadenosine, a concentration which completely inhibited the growth of non-transgenic cultures. ADA activity appeared to segregate in progency of regenerated plants as a single, dominant Mendelian trait. These results suggest that ADA, in combination with adenosine analogue selection, represents a potentially viable selectable marker system for transgenic maize production.

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