Retinoic Acid Delays Transcription of Human Retinal Pigment Neuroepithelium Marker Genes in ARPE-19 Cells

Overview

Journal Neuroreport

Specialty Neurology

Date 2000 Jun 7

PMID 10841379

Citations 9

Authors

J J Janssen

E D Kuhlmann

A H Van Vugt

H J Winkens

B P Janssen

A F Deutman

C A Driessen

Affiliations

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Abstract

The effect of retinoic acid on the differentiation of a human retinal pigment epithelium-derived cell line ARPE-19 was studied. Differentiation of ARPE-19 cells is delayed by retinoic acid. The minimum all-trans-retinoic acid concentration needed for delay of ARPE-19 differentiation is 1 microM. A delay of differentiation was also observed using 1 microM 9-cis or 13-cis-retinoic acid. Differentiation at the molecular level was studied by analyzing transcription of two RPE-marker genes, RPE65 and peropsin. In the presence of 1 microM retinoic acid the onset of transcription of both genes was delayed by 2-3 weeks. We conclude that all-trans-, 9-cis-, and 13-cis-retinoic acid delay differentiation of ARPE-19 cells into cells that phenotypically resemble cells from the human retinal pigment epithelium.

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