Biosynthesis of the Lantibiotic Mersacidin: Organization of a Type B Lantibiotic Gene Cluster

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2000 Jun 1

PMID 10831439

Citations 59

Authors

K Altena

A Guder

C Cramer

G Bierbaum

Affiliations

Soon will be listed here.

Abstract

The biosynthetic gene cluster (12.3 kb) of mersacidin, a lanthionine-containing antimicrobial peptide, is located on the chromosome of the producer, Bacillus sp. strain HIL Y-85,54728 in a region that corresponds to 348 degrees on the chromosome of Bacillus subtilis 168. It consists of 10 open reading frames and contains, in addition to the previously described mersacidin structural gene mrsA (G. Bierbaum, H. Brötz, K.-P. Koller, and H.-G. Sahl, FEMS Microbiol. Lett. 127:121-126, 1995), two genes, mrsM and mrsD, coding for enzymes involved in posttranslational modification of the prepeptide; one gene, mrsT, coding for a transporter with an associated protease domain; and three genes, mrsF, mrsG, and mrsE, encoding a group B ABC transporter that could be involved in producer self-protection. Additionally, three regulatory genes are part of the gene cluster, i.e., mrsR2 and mrsK2, which encode a two-component regulatory system which seems to be necessary for the transcription of the mrsFGE operon, and mrsR1, which encodes a protein with similarity to response regulators. Transcription of mrsA sets in at early stationary phase (between 8 and 16 h of culture).

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References

Havarstein L, Diep D, Nes I . A family of bacteriocin ABC transporters carry out proteolytic processing of their substrates concomitant with export. Mol Microbiol. 1995; 16(2):229-40. DOI: 10.1111/j.1365-2958.1995.tb02295.x. View

Kuipers O, Beerthuyzen M, de Ruyter P, Luesink E, de Vos W . Autoregulation of nisin biosynthesis in Lactococcus lactis by signal transduction. J Biol Chem. 1995; 270(45):27299-304. DOI: 10.1074/jbc.270.45.27299. View

Bisschop A, Konings W . Reconstitution of reduced nicotinamide adenine dinucleotide oxidase activity with menadione in membrane vesicles from the menaquinone-deficient Bacillus subtilis aro D. Relation between electron transfer and active transport. Eur J Biochem. 1976; 67(2):357-65. DOI: 10.1111/j.1432-1033.1976.tb10699.x. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Gryczan T, Hahn J, Contente S, Dubnau D . Replication and incompatibility properties of plasmid pE194 in Bacillus subtilis. J Bacteriol. 1982; 152(2):722-35. PMC: 221522. DOI: 10.1128/jb.152.2.722-735.1982. View

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View

Vieira J, Messing J . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985; 33(1):103-19. DOI: 10.1016/0378-1119(85)90120-9. View

Pearson W, Lipman D . Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988; 85(8):2444-8. PMC: 280013. DOI: 10.1073/pnas.85.8.2444. View

Schnell N, Entian K, Schneider U, Gotz F, Zahner H, Kellner R . Prepeptide sequence of epidermin, a ribosomally synthesized antibiotic with four sulphide-rings. Nature. 1988; 333(6170):276-8. DOI: 10.1038/333276a0. View

10.

Kraft R, Tardiff J, Krauter K, Leinwand L . Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase. Biotechniques. 1988; 6(6):544-6, 549. View

11.

Augustin J, Rosenstein R, Wieland B, Schneider U, Schnell N, Engelke G . Genetic analysis of epidermin biosynthetic genes and epidermin-negative mutants of Staphylococcus epidermidis. Eur J Biochem. 1992; 204(3):1149-54. DOI: 10.1111/j.1432-1033.1992.tb16740.x. View

12.

Chatterjee S, Lad S, Phansalkar M, Rupp R, Ganguli B, Fehlhaber H . Mersacidin, a new antibiotic from Bacillus. Fermentation, isolation, purification and chemical characterization. J Antibiot (Tokyo). 1992; 45(6):832-8. DOI: 10.7164/antibiotics.45.832. View

13.

Chatterjee S, Chatterjee D, Jani R, Blumbach J, Ganguli B, Klesel N . Mersacidin, a new antibiotic from Bacillus. In vitro and in vivo antibacterial activity. J Antibiot (Tokyo). 1992; 45(6):839-45. DOI: 10.7164/antibiotics.45.839. View

14.

Liu W, Hansen J . Enhancement of the chemical and antimicrobial properties of subtilin by site-directed mutagenesis. J Biol Chem. 1992; 267(35):25078-85. View

15.

Klein C, Kaletta C, Entian K . Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system. Appl Environ Microbiol. 1993; 59(1):296-303. PMC: 202094. DOI: 10.1128/aem.59.1.296-303.1993. View

16.

van der Meer J, Polman J, Beerthuyzen M, Siezen R, Kuipers O, de Vos W . Characterization of the Lactococcus lactis nisin A operon genes nisP, encoding a subtilisin-like serine protease involved in precursor processing, and nisR, encoding a regulatory protein involved in nisin biosynthesis. J Bacteriol. 1993; 175(9):2578-88. PMC: 204559. DOI: 10.1128/jb.175.9.2578-2588.1993. View

17.

Kuipers O, Beerthuyzen M, Siezen R, de Vos W . Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis. Requirement of expression of the nisA and nisI genes for development of immunity. Eur J Biochem. 1993; 216(1):281-91. DOI: 10.1111/j.1432-1033.1993.tb18143.x. View

18.

Peschel A, Augustin J, Kupke T, Stevanovic S, Gotz F . Regulation of epidermin biosynthetic genes by EpiQ. Mol Microbiol. 1993; 9(1):31-9. DOI: 10.1111/j.1365-2958.1993.tb01666.x. View

19.

Feliciello I, Chinali G . A modified alkaline lysis method for the preparation of highly purified plasmid DNA from Escherichia coli. Anal Biochem. 1993; 212(2):394-401. DOI: 10.1006/abio.1993.1346. View

20.

van der Meer J, Rollema H, Siezen R, Beerthuyzen M, Kuipers O, de Vos W . Influence of amino acid substitutions in the nisin leader peptide on biosynthesis and secretion of nisin by Lactococcus lactis. J Biol Chem. 1994; 269(5):3555-62. View