Deficiency of the Macrophage Migration Inhibitory Factor Gene Has No Significant Effect on Endotoxaemia

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 2000 May 16

PMID 10809963

Citations 15

Authors

N Honma

H Koseki

T Akasaka

T Nakayama

M Taniguchi

I Serizawa

H Akahori

M Osawa

T Mikayama

Affiliations

Soon will be listed here.

Abstract

By targeted disruption of the MIF gene, we have established a mouse strain deficient in macrophage (Mphi) migration inhibitory factor (MIF). Despite previous reports indicating an essential role of MIF in endotoxaemia, an injection of lipopolysaccharide (LPS) into the MIF-deficient mice (maintained under specific pathogen-free conditions) caused shock. No significant difference was detected between the MIF-deficient mutant and normal mice in susceptibility to LPS for endotoxaemia or tumour necrosis factor-alpha (TNF-alpha) formation upon LPS injection. Peritoneal Mphi from the two strains produced TNF-alpha in response to LPS with similar dose responses. Dexamethasone suppressed the LPS-induced TNF-alpha response of Mphi, but no difference was detected between the Mphi from the two strains. These results suggest that endogenous MIF has no significant effect on the LPS-induced TNF-alpha production and no effect on suppression of the response by glucocorticoids. Thus, MIF is not crucial for LPS-induced immune responses leading to shock.

Citing Articles

Role of macrophage migration inhibitory factor (MIF) in pollen-induced allergic conjunctivitis and pollen dermatitis in mice.

Nagata Y, Yoshihisa Y, Matsunaga K, Rehman M, Kitaichi N, Kitaichi N PLoS One. 2015; 10(2):e0115593.

PMID: 25647395 PMC: 4315585. DOI: 10.1371/journal.pone.0115593.

Macrophage migration inhibitory factor is essential for osteoclastogenic mechanisms in vitro and in vivo mouse model of arthritis.

Gu R, Santos L, Ngo D, Fan H, Singh P, Fingerle-Rowson G Cytokine. 2015; 72(2):135-45.

PMID: 25647268 PMC: 4355090. DOI: 10.1016/j.cyto.2014.11.015.

Deletion of macrophage migration inhibitory factor worsens stroke outcome in female mice.

Turtzo L, Li J, Persky R, Benashski S, Weston G, Bucala R Neurobiol Dis. 2013; 54:421-31.

PMID: 23376686 PMC: 3628925. DOI: 10.1016/j.nbd.2013.01.016.

Brain miffed by macrophage migration inhibitory factor.

Savaskan N, Fingerle-Rowson G, Buchfelder M, Eyupoglu I Int J Cell Biol. 2012; 2012:139573.

PMID: 22973314 PMC: 3438795. DOI: 10.1155/2012/139573.

Macrophage migration inhibitory factor produced by the tumour stroma but not by tumour cells regulates angiogenesis in the B16-F10 melanoma model.

Girard E, Strathdee C, Trueblood E, Queva C Br J Cancer. 2012; 107(9):1498-505.

PMID: 22955855 PMC: 3493755. DOI: 10.1038/bjc.2012.392.

References

Bloom B, Bennett B . Mechanism of a reaction in vitro associated with delayed-type hypersensitivity. Science. 1966; 153(3731):80-2. DOI: 10.1126/science.153.3731.80. View

Nagy A, Rossant J, Nagy R, Abramow-Newerly W, Roder J . Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. Proc Natl Acad Sci U S A. 1993; 90(18):8424-8. PMC: 47369. DOI: 10.1073/pnas.90.18.8424. View

Rocklin R, Rosen F, David J . In vitro lymphocyte response of patients with immunologic deficiency diseases. N Engl J Med. 1970; 282(24):1340-3. DOI: 10.1056/NEJM197006112822404. View

David J, DAVID R . Cellular hypersensitivity and immunity. Inhibition of macrophage migration and the lymphocyte mediators. Prog Allergy. 1972; 16:300-449. View

Beutler B, Milsark I, Cerami A . Passive immunization against cachectin/tumor necrosis factor protects mice from lethal effect of endotoxin. Science. 1985; 229(4716):869-71. DOI: 10.1126/science.3895437. View

Gifford G, Lohmann-Matthes M . Requirement for the continual presence of lipopolysaccharide for production of tumor necrosis factor by thioglycollate-induced peritoneal murine macrophages. Int J Cancer. 1986; 38(1):135-7. DOI: 10.1002/ijc.2910380121. View

NEEDLEMAN P, Turk J, Jakschik B, Morrison A, Lefkowith J . Arachidonic acid metabolism. Annu Rev Biochem. 1986; 55:69-102. DOI: 10.1146/annurev.bi.55.070186.000441. View

Tracey K, Fong Y, Hesse D, Manogue K, Lee A, Kuo G . Anti-cachectin/TNF monoclonal antibodies prevent septic shock during lethal bacteraemia. Nature. 1987; 330(6149):662-4. DOI: 10.1038/330662a0. View

Bernhagen J, Calandra T, Mitchell R, Martin S, Tracey K, Voelter W . MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia. Nature. 1993; 365(6448):756-9. DOI: 10.1038/365756a0. View

10.

Mikayama T, Nakano T, Gomi H, Nakagawa Y, Liu Y, Sato M . Molecular cloning and functional expression of a cDNA encoding glycosylation-inhibiting factor. Proc Natl Acad Sci U S A. 1993; 90(21):10056-60. PMC: 47712. DOI: 10.1073/pnas.90.21.10056. View

11.

Calandra T, Bernhagen J, Mitchell R, Bucala R . The macrophage is an important and previously unrecognized source of macrophage migration inhibitory factor. J Exp Med. 1994; 179(6):1895-902. PMC: 2191507. DOI: 10.1084/jem.179.6.1895. View

12.

Bernhagen J, Calandra T, Cerami A, Bucala R . Macrophage migration inhibitory factor is a neuroendocrine mediator of endotoxaemia. Trends Microbiol. 1994; 2(6):198-201. DOI: 10.1016/0966-842x(94)90111-h. View

13.

Bernhagen J, Mitchell R, Calandra T, Voelter W, Cerami A, Bucala R . Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF). Biochemistry. 1994; 33(47):14144-55. DOI: 10.1021/bi00251a025. View

14.

Mitchell R, Bacher M, Bernhagen J, Pushkarskaya T, Seldin M, Bucala R . Cloning and characterization of the gene for mouse macrophage migration inhibitory factor (MIF). J Immunol. 1995; 154(8):3863-70. View

15.

RANDOW F, Syrbe U, Meisel C, Krausch D, Zuckermann H, Platzer C . Mechanism of endotoxin desensitization: involvement of interleukin 10 and transforming growth factor beta. J Exp Med. 1995; 181(5):1887-92. PMC: 2191997. DOI: 10.1084/jem.181.5.1887. View

16.

Calandra T, Bernhagen J, Metz C, SPIEGEL L, Bacher M, Donnelly T . MIF as a glucocorticoid-induced modulator of cytokine production. Nature. 1995; 377(6544):68-71. DOI: 10.1038/377068a0. View

17.

BOZZA M, Kolakowski Jr L, Jenkins N, Gilbert D, Copeland N, David J . Structural characterization and chromosomal location of the mouse macrophage migration inhibitory factor gene and pseudogenes. Genomics. 1995; 27(3):412-9. DOI: 10.1006/geno.1995.1071. View

18.

Bernhagen J, Calandra T, Bucala R . The emerging role of MIF in septic shock and infection. Biotherapy. 1994; 8(2):123-7. DOI: 10.1007/BF01878495. View

19.

ROSENGREN E, Bucala R, Aman P, Jacobsson L, Odh G, Metz C . The immunoregulatory mediator macrophage migration inhibitory factor (MIF) catalyzes a tautomerization reaction. Mol Med. 1996; 2(1):143-9. PMC: 2230029. View

20.

Honma N, Matsuda Y, Ishii Y, Sato M, Tomura T, Yuyama N . Cloning and mapping of the mouse glycosylation-inhibiting factor gene and four related genes. Mamm Genome. 1996; 7(1):59-61. DOI: 10.1007/s003359900014. View