Hybrid Isolation by Recovery of RNA-DNA Hybrids from Agar Using S1 Nuclease

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1974 Jun 1

PMID 10793757

Citations 3

Authors

J N Hansen

B H Pheiffer

C J Hough

Affiliations

Soon will be listed here.

Abstract

A method for recovering RNA-DNA hybrids from agar employing a single strand specific nuclease is described. The procedure is suitable for large scale isolations, and immobilization of the DNA in agar prior to hybridization allows a high yield of hybrid without interference by DNA reannealing.

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References

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