Heterologous Mischarging As a Means of TRNA Fractionation. I. Behaviour of E. Coli Phenylalanyl-tRNA(1Val) on BD-cellulose

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1974 Aug 1

PMID 10793726

Citations 1

Authors

E Rosenmann

M Krauskopf

Affiliations

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Abstract

The chromatographic behaviour of E.coli tRNA(Val) (1), Val-tRNA(Val) (1) and Phe-tRNA(Val) (1) was studied on BD-cellulose columns. At pH 4.0 and 4 degrees C the elution position of Phe-tRNA(Val) (1) was not affected by the presence of absence of Mg(2+), whereas Val-tRNA(Val) (1) was slightly retarded when Mg(2+) was ommited. It is postulated that the amino acid and its nature influence the structure that the aminoacyl-tRNA assumes. Under suitable conditions the heteroaminoacylated Phe-tRNA(Val) (1) eluted significantly later than other tRNAs. This fact showed that heterologous mischarging can be a useful step in tRNA purification methods.

Citing Articles

Heterologous mischarging as a means of tRNA fractionation. II. Isolation of E. coli tRNA1Val and tRNA1Ala.

Leon G, Ruiz N, Meza L, Krauskopf M Experientia. 1977; 33(8):1029-30.

PMID: 142648 DOI: 10.1007/BF01945950.

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