Design and Evaluation of a Lactobacillus Manihotivorans Species-specific RRNA-targeted Hybridization Probe and Its Application to the Study of Sour Cassava Fermentation

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2000 May 2

PMID 10788405

Authors

F Ampe

Affiliations

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Abstract

Based on 16S rRNA sequence comparison, we have designed a 20-mer oligonucleotide that targets a region specific to the species Lactobacillus manihotivorans recently isolated from sour cassava fermentation. The probe recognized the rRNA obtained from all the L. manihotivorans strains tested but did not recognize 56 strains of microorganisms from culture collections or directly isolated from sour cassava, including 29 species of lactic acid bacteria. This probe was then successfully used in quantitative RNA blots and demonstrated the importance of L. manihotivorans in the fermentation of sour cassava starch, which could represent up to 20% of total lactic acid bacteria.

References

Dore J, Sghir A, Corthier G, Pochart P . Design and evaluation of a 16S rRNA-targeted oligonucleotide probe for specific detection and quantitation of human faecal Bacteroides populations. Syst Appl Microbiol. 1998; 21(1):65-71. DOI: 10.1016/S0723-2020(98)80009-X. View

Ampe F, Omar N, Guyot J . Recovery of total microbial RNA from lactic acid fermented foods with a high starch content. Lett Appl Microbiol. 1998; 27(5):270-4. DOI: 10.1046/j.1472-765x.1998.t01-7-00449.x. View

Maidak B, Cole J, Parker Jr C, Garrity G, Larsen N, Li B . A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res. 1998; 27(1):171-3. PMC: 148126. DOI: 10.1093/nar/27.1.171. View

Zheng D, Alm E, Stahl D, Raskin L . Characterization of universal small-subunit rRNA hybridization probes for quantitative molecular microbial ecology studies. Appl Environ Microbiol. 1996; 62(12):4504-13. PMC: 168277. DOI: 10.1128/aem.62.12.4504-4513.1996. View

Morlon-Guyot J, Guyot J, Pot B, Jacobe de Haut I, Raimbault M . Lactobacillus manihotivorans sp. nov., a new starch-hydrolysing lactic acid bacterium isolated during cassava sour starch fermentation. Int J Syst Bacteriol. 1998; 48 Pt 4:1101-9. DOI: 10.1099/00207713-48-4-1101. View

Lin C, Stahl D . Taxon-specific probes for the cellulolytic genus Fibrobacter reveal abundant and novel equine-associated populations. Appl Environ Microbiol. 1995; 61(4):1348-51. PMC: 167390. DOI: 10.1128/aem.61.4.1348-1351.1995. View

Stahl D, Flesher B, MANSFIELD H, Montgomery L . Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology. Appl Environ Microbiol. 1988; 54(5):1079-84. PMC: 202606. DOI: 10.1128/aem.54.5.1079-1084.1988. View

Ampe F, Omar N, Guyot J . Culture-independent quantification of physiologically-active microbial groups in fermented foods using rRNA-targeted oligonucleotide probes: application to pozol, a Mexican lactic acid fermented maize dough. J Appl Microbiol. 1999; 87(1):131-40. DOI: 10.1046/j.1365-2672.1999.00803.x. View

Ampe F, Omar N, Moizan C, Wacher C, Guyot J . Polyphasic study of the spatial distribution of microorganisms in Mexican pozol, a fermented maize dough, demonstrates the need for cultivation-independent methods to investigate traditional fermentations. Appl Environ Microbiol. 1999; 65(12):5464-73. PMC: 91745. DOI: 10.1128/AEM.65.12.5464-5473.1999. View

10.

Brauman A, Keleke S, Malonga M, Miambi E, Ampe F . Microbiological and biochemical characterization of cassava retting, a traditional lactic Acid fermentation for foo-foo (cassava flour) production. Appl Environ Microbiol. 1996; 62(8):2854-8. PMC: 1388916. DOI: 10.1128/aem.62.8.2854-2858.1996. View

11.

Sghir A, Antonopoulos D, Mackie R . Design and evaluation of a Lactobacillus group-specific ribosomal RNA-targeted hybridization probe and its application to the study of intestinal microecology in pigs. Syst Appl Microbiol. 1998; 21(2):291-6. DOI: 10.1016/S0723-2020(98)80036-2. View

12.

Poulsen L, Ballard G, Stahl D . Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl Environ Microbiol. 1993; 59(5):1354-60. PMC: 182089. DOI: 10.1128/aem.59.5.1354-1360.1993. View

13.

Alm E, Oerther D, Larsen N, Stahl D, Raskin L . The oligonucleotide probe database. Appl Environ Microbiol. 1996; 62(10):3557-9. PMC: 168159. DOI: 10.1128/aem.62.10.3557-3559.1996. View