Involvement of NRAMP1 from Arabidopsis Thaliana in Iron Transport

Overview

Journal Biochem J

Specialty Biochemistry

Date 2000 Apr 19

PMID 10769179

Citations 145

Authors

C Curie

J M Alonso

M Le Jean

J R Ecker

J F Briat

Affiliations

Soon will be listed here.

Abstract

Nramp genes code for a widely distributed class of proteins involved in a variety of processes, ranging from the control of susceptibility to bacterial infection in mammalian cells and taste behaviour in Drosophila to manganese uptake in yeast. Some of the NRAMP proteins in mammals and in yeast are capable of transporting metal ions, including iron. In plants, iron transport was shown to require a reduction/Fe(II) transport system. In Arabidopsis thaliana this process involves the IRT1 and Fro2 genes. Here we report the sequence of five NRAMP proteins from A. thaliana. Sequence comparison suggests that there are two classes of NRAMP proteins in plants: A. thaliana (At) NRAMP1 and Oriza sativa (Os) NRAMP1 and 3 (two rice isologues) represent one class, and AtNRAMP2-5 and OsNRAMP2 the other. AtNramp1 and OsNramp1 are able to complement the fet3fet4 yeast mutant defective both in low- and high-affinity iron transports, whereas AtNramp2 and OsNramp2 fail to do so. In addition, AtNramp1 transcript, but not AtNramp2 transcript, accumulates in response to iron deficiency in roots but not in leaves. Finally, overexpression of AtNramp1 in transgenic A. thaliana plants leads to an increase in plant resistance to toxic iron concentration. Taken together, these results demonstrate that AtNramp1 participates in the control of iron homoeostasis in plants.

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References

Fox T, Shaff J, Grusak M, Norvell W, Chen Y, Chaney R . Direct Measurement of 59Fe-Labeled Fe2+ Influx in Roots of Pea Using a Chelator Buffer System to Control Free Fe2+ in Solution. Plant Physiol. 1996; 111(1):93-100. PMC: 157815. DOI: 10.1104/pp.111.1.93. View

Yi Y, Guerinot M . Genetic evidence that induction of root Fe(III) chelate reductase activity is necessary for iron uptake under iron deficiency. Plant J. 1996; 10(5):835-44. DOI: 10.1046/j.1365-313x.1996.10050835.x. View

Brunelli J, Pall M . A series of yeast/Escherichia coli lambda expression vectors designed for directional cloning of cDNAs and cre/lox-mediated plasmid excision. Yeast. 1993; 9(12):1309-18. DOI: 10.1002/yea.320091204. View

Cellier M, Belouchi A, Gros P . Resistance to intracellular infections: comparative genomic analysis of Nramp. Trends Genet. 1996; 12(6):201-4. DOI: 10.1016/0168-9525(96)30042-5. View

Pinner E, Gruenheid S, Raymond M, Gros P . Functional complementation of the yeast divalent cation transporter family SMF by NRAMP2, a member of the mammalian natural resistance-associated macrophage protein family. J Biol Chem. 1997; 272(46):28933-8. DOI: 10.1074/jbc.272.46.28933. View

Radisky D, Babcock M, Kaplan J . The yeast frataxin homologue mediates mitochondrial iron efflux. Evidence for a mitochondrial iron cycle. J Biol Chem. 1999; 274(8):4497-9. DOI: 10.1074/jbc.274.8.4497. View

Chen X, Peng J, Cohen A, Nelson H, Nelson N, Hediger M . Yeast SMF1 mediates H(+)-coupled iron uptake with concomitant uncoupled cation currents. J Biol Chem. 1999; 274(49):35089-94. DOI: 10.1074/jbc.274.49.35089. View

Fleming M, Trenor 3rd C, Su M, Foernzler D, Beier D, Dietrich W . Microcytic anaemia mice have a mutation in Nramp2, a candidate iron transporter gene. Nat Genet. 1997; 16(4):383-6. DOI: 10.1038/ng0897-383. View

Eide D, Broderius M, Fett J, Guerinot M . A novel iron-regulated metal transporter from plants identified by functional expression in yeast. Proc Natl Acad Sci U S A. 1996; 93(11):5624-8. PMC: 39298. DOI: 10.1073/pnas.93.11.5624. View

10.

Eide D . The molecular biology of metal ion transport in Saccharomyces cerevisiae. Annu Rev Nutr. 1998; 18:441-69. DOI: 10.1146/annurev.nutr.18.1.441. View

11.

Liu X, Culotta V . Mutational analysis of Saccharomyces cerevisiae Smf1p, a member of the Nramp family of metal transporters. J Mol Biol. 1999; 289(4):885-91. DOI: 10.1006/jmbi.1999.2815. View

12.

Vidal S, Malo D, Vogan K, Skamene E, Gros P . Natural resistance to infection with intracellular parasites: isolation of a candidate for Bcg. Cell. 1993; 73(3):469-85. DOI: 10.1016/0092-8674(93)90135-d. View

13.

Reuber T, Ausubel F . Isolation of Arabidopsis genes that differentiate between resistance responses mediated by the RPS2 and RPM1 disease resistance genes. Plant Cell. 1996; 8(2):241-9. PMC: 161094. DOI: 10.1105/tpc.8.2.241. View

14.

Fobis-Loisy I, Loridon K, Lobreaux S, Lebrun M, Briat J . Structure and differential expression of two maize ferritin genes in response to iron and abscisic acid. Eur J Biochem. 1995; 231(3):609-19. DOI: 10.1111/j.1432-1033.1995.tb20739.x. View

15.

Liu X, Culotta V . Post-translation control of Nramp metal transport in yeast. Role of metal ions and the BSD2 gene. J Biol Chem. 1999; 274(8):4863-8. DOI: 10.1074/jbc.274.8.4863. View

16.

DSouza J, Cheah P, Gros P, Chia W, Rodrigues V . Functional complementation of the malvolio mutation in the taste pathway of Drosophila melanogaster by the human natural resistance-associated macrophage protein 1 (Nramp-1). J Exp Biol. 1999; 202(Pt 14):1909-15. DOI: 10.1242/jeb.202.14.1909. View

17.

Alonso J, Hirayama T, Roman G, Nourizadeh S, Ecker J . EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis. Science. 1999; 284(5423):2148-52. DOI: 10.1126/science.284.5423.2148. View

18.

Raguzzi F, Lesuisse E, Crichton R . Iron storage in Saccharomyces cerevisiae. FEBS Lett. 1988; 231(1):253-8. DOI: 10.1016/0014-5793(88)80742-7. View

19.

Robinson N, Procter C, Connolly E, Guerinot M . A ferric-chelate reductase for iron uptake from soils. Nature. 1999; 397(6721):694-7. DOI: 10.1038/17800. View

20.

Belouchi A, Kwan T, Gros P . Cloning and characterization of the OsNramp family from Oryza sativa, a new family of membrane proteins possibly implicated in the transport of metal ions. Plant Mol Biol. 1997; 33(6):1085-92. DOI: 10.1023/a:1005723304911. View