The Human DINB1 Gene Encodes the DNA Polymerase Poltheta

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2000 Apr 13

PMID 10760255

Citations 70

Authors

R E Johnson

S Prakash

L Prakash

Affiliations

Soon will be listed here.

Abstract

The human DINB1 gene shares a high degree of homology with the Escherichia coli dinB gene. Here, we purify the hDINB1-encoded protein and show that it is a DNA polymerase. Because hDinB1 is the eighth eukaryotic DNA polymerase to be described, we have named it DNA polymerase (Pol) theta. hPoltheta is unable to bypass a cis-syn thymine-thymine dimer, nor does it bypass a (6-4) photoproduct or an abasic site. We also examine the fidelity of hPoltheta on nondamaged DNA templates by steady-state kinetic analyses and find that hPoltheta misincorporates deoxynucleotides with a frequency of about 10(-3) to 10(-4). We discuss the relationship between the fidelity of hPoltheta and its inability to bypass DNA damage.

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