Photo-control of Helix Content in a Short Peptide

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2000 Apr 13

PMID 10760254

Citations 53

Authors

J R Kumita

O S Smart

G A Woolley

Affiliations

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Abstract

The alpha-helix is a key structural element in a wide range of peptides and proteins. We report here the design, synthesis, and characterization of a modified peptide in which the helix content can be reversibly photoregulated. The peptide contains two cysteine residues that are cross-linked by an azobenzene derivative in an intramolecular fashion. In accordance with the design, the photoisomerization of the azobenzene cross-linker from the trans to the cis form causes a large increase in the helix content of the peptide, in water.

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