Determination of Antibody Levels to Candida Albicans in Healthy and Hospitalised Adults Using a Radioimmunoassay

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1978 Dec 1

PMID 107198

Citations 9

Authors

S J Cobb

D Parratt

Affiliations

Soon will be listed here.

Abstract

A radioimmunoassay for antibody to Candida albicans is described. The test used whole, killed organisms as the antigen and radiolabelled sheep anti-human globulins to quantitate different classes of antibody to C. albicans. The assay has been compared with an Ouchterlony precipitin method and found to be simpler, more rapid, and more sensitive than the latter. Results obtained from two groups of symptomless adults indicated that the range of antibody level was wider for a hospitalised group than for a group of blood transfusion donors, particularly in respect of IgG and IgA antibody. The reason for the increase of antibody in hospital patients was not clear but may have been related to antibiotic therapy. The difficulties in interpretation of Candida serology have therefore been re-assessed in the light of more detailed knowledge of the range and type of antibody to be expected in normal individuals.

Citing Articles

The doctor's dilemma: systemic candidiasis.

McDonnell L, Farrell M Ir J Med Sci. 1981; 150(1):15-7.

PMID: 7216712 DOI: 10.1007/BF02938187.

Quantitation of antibody against cell wall mannan and a major cytoplasmic antigen of Candida in rabbits, mice, and humans.

Jones J Infect Immun. 1980; 30(1):78-89.

PMID: 7002793 PMC: 551280. DOI: 10.1128/iai.30.1.78-89.1980.

Demonstration and solubilization of antigens expressed primarily on the surfaces of Candida albicans germ tubes.

Smail E, Jones J Infect Immun. 1984; 45(1):74-81.

PMID: 6376363 PMC: 263269. DOI: 10.1128/iai.45.1.74-81.1984.

Further evaluation of factors affecting a rapid ELISA procedure for detection of IgG antibodies to Candida albicans.

Richardson M, Smith S, Warnock D Mycopathologia. 1983; 82(3):167-73.

PMID: 6350883 DOI: 10.1007/BF00439222.

Estimations of serum arabinitol for diagnosing invasive candidosis.

Deacon A J Clin Pathol. 1986; 39(8):842-50.

PMID: 3745474 PMC: 500106. DOI: 10.1136/jcp.39.8.842.

References

STALLYBRASS F . CANDIDA PRECIPITINS. J Pathol Bacteriol. 1964; 87:89-97. DOI: 10.1002/path.1700870113. View

McCONAHEY P, Dixon F . A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966; 29(2):185-9. DOI: 10.1159/000229699. View

Hollingdale M . Antibody responses in patients with farmer's lung disease to antigens from Micropolyspora faeni. J Hyg (Lond). 1974; 72(1):79-89. PMC: 2130260. DOI: 10.1017/s002217240002324x. View

Axelsen N, Kirkpatrick C, Buckley R . Precipitins to Candida albicans in chronic mucocutaneous candidiasis studied by crossed immunoelectrophoresis with intermediate gel. Correlation with clinical and immunological findings. Clin Exp Immunol. 1974; 17(3):385-94. PMC: 1554077. View

Nielsen K, Parratt D, White R . Quantitation of antibody to particulate antigens using a radiolabelled anti-immunoglobulin reagent: application to estimation of antibody in farmer's lung syndrome. J Immunol Methods. 1973; 3(3):301-13. DOI: 10.1016/0022-1759(73)90025-2. View

Preisler H, Hasenclever H, Levitan A, HENDERSON E . Serologic diagnosis of disseminated candidiasis in patients with acute leukemia. Ann Intern Med. 1969; 70(1):19-30. DOI: 10.7326/0003-4819-70-1-19. View

Seelig M . The role of antibiotics in the pathogenesis of Candidainfections. Am J Med. 1966; 40(6):887-917. DOI: 10.1016/0002-9343(66)90204-x. View

Lehner T . Immunofluorescence study of Candida albicans in candidiasis, carriers and controls. J Pathol Bacteriol. 1966; 91(1):97-104. DOI: 10.1002/path.1700910114. View

Winner H . The transition from commensalism to parasitism. Br J Dermatol. 1969; 81:Suppl 1:62+. DOI: 10.1111/j.1365-2133.1969.tb12835.x. View

10.

CHEW W, Theus T . Candida precipitins. J Immunol. 1967; 98(2):220-4. View

11.

Axelsen N . Analysis of human Candida precipitins by quantitative immunoelectrophoresis: a model for analysis of complex microbial antigen-antibody systems. Scand J Immunol. 1976; 5(3):177-90. DOI: 10.1111/j.1365-3083.1976.tb00268.x. View

12.

Ho Y, Ng M, TEOH-CHAN C, Yue P, Huang C . Indirect immunofluorescence assay for antibody to germ tube of Candida albicans--a new diagnostic test. J Clin Pathol. 1976; 29(11):1007-10. PMC: 476260. DOI: 10.1136/jcp.29.11.1007. View

13.

Odds F, EVANS E, Holland K . Detection of Candida precipitins. a comparison of double diffusion and counter immunoelectrophoresis. J Immunol Methods. 1975; 7(2-3):211-8. DOI: 10.1016/0022-1759(75)90018-6. View

14.

Parratt D, Nielsen K, Boyd G, White R . The quantitation of antibody in farmer's lung syndrome using a radioimmunoassay. Results of a clinical survey and comparison of three serological methods. Clin Exp Immunol. 1975; 20(2):217-25. PMC: 1538190. View