» Articles » PMID: 10707951

Rapid Discrimination Between Methicillin-sensitive and Methicillin-resistant Staphylococcus Aureus by Intact Cell Mass Spectrometry

Overview
Journal J Med Microbiol
Specialty Microbiology
Date 2000 Mar 9
PMID 10707951
Citations 61
Authors
Affiliations
Soon will be listed here.
Abstract

Rapid, accurate discrimination between methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) strains is essential for appropriate therapeutic management and timely intervention for infection control. A rapid method involving intact cell mass spectrometry (ICMS) is presented that shows promise for identification, discrimination of MSSA from MRSA and typing. In ICMS, cells from a bacterial colony are emulsified in a chemical matrix, added to a sample slide, dried and analysed by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS). This technique examines the chemistry of the intact bacterial cell surface, yielding spectra consisting of a series of peaks from 500 to 10000, which represent the mass:charge (m:z) ratios. Each peak corresponds to a molecular fragment released from the cell surface during laser desorption. Specimens can be prepared in a few seconds from plate cultures and a spectrum can be obtained within 2 min. ICMS spectra for 20 staphylococcal isolates showed characteristic peaks, some of which were conserved at species level, some at strain level and some were characteristic of the methicillin susceptibility status of the strain. ICMS may have potential for MRSA identification and typing, and may improve infection control measures.

Citing Articles

Identification of Legionella by MALDI Biotyper through three preparation methods and an in-house library comparing phylogenetic and hierarchical cluster results.

Girolamini L, Caiazza P, Marino F, Pascale M, Caligaris L, Spiteri S Sci Rep. 2025; 15(1):2162.

PMID: 39820522 PMC: 11739463. DOI: 10.1038/s41598-025-85251-4.


Metabolic insights from mass spectrometry imaging of biofilms: A perspective from model microorganisms.

Parmar D, Rosado-Rosa J, Shrout J, Sweedler J Methods. 2024; 224:21-34.

PMID: 38295894 PMC: 11149699. DOI: 10.1016/j.ymeth.2024.01.014.


Nano-Biotechnology for Bacteria Identification and Potent Anti-bacterial Properties: A Review of Current State of the Art.

Kaushal S, Priyadarshi N, Garg P, Singhal N, Lim D Nanomaterials (Basel). 2023; 13(18).

PMID: 37764558 PMC: 10536455. DOI: 10.3390/nano13182529.


Using Protein Fingerprinting for Identifying and Discriminating Methicillin Resistant Isolates from Inpatient and Outpatient Clinics.

Elbehiry A, Marzouk E, Moussa I, Anagreyyah S, Alghamdi A, Alqarni A Diagnostics (Basel). 2023; 13(17).

PMID: 37685363 PMC: 10486511. DOI: 10.3390/diagnostics13172825.


Evaluation of an adapted method of relative growth to determine the susceptibility of Enterobacterales to polymyxin B by MALDI-TOF MS.

Inamine E, Carneiro M, Wilhelm C, Barth A Braz J Microbiol. 2023; 54(3):1841-1846.

PMID: 37402940 PMC: 10484837. DOI: 10.1007/s42770-023-01014-1.