Schwann Cell Myelination Requires Timely and Precise Targeting of P(0) Protein

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2000 Mar 8

PMID 10704450

Citations 27

Authors

X Yin

G J Kidd

L Wrabetz

M L Feltri

A Messing

B D Trapp

Affiliations

Soon will be listed here.

Abstract

This report investigated mechanisms responsible for failed Schwann cell myelination in mice that overexpress P(0) (P(0)(tg)), the major structural protein of PNS myelin. Quantitative ultrastructural immunocytochemistry established that P(0) protein was mistargeted to abaxonal, periaxonal, and mesaxon membranes in P(0)(tg) Schwann cells with arrested myelination. The extracellular leaflets of P(0)-containing mesaxon membranes were closely apposed with periodicities of compact myelin. The myelin-associated glycoprotein was appropriately sorted in the Golgi apparatus and targeted to periaxonal membranes. In adult mice, occasional Schwann cells myelinated axons possibly with the aid of endocytic removal of mistargeted P(0). These results indicate that P(0) gene multiplication causes P(0) mistargeting to mesaxon membranes, and through obligate P(0) homophilic adhesion, renders these dynamic membranes inert and halts myelination.

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