Preparation and Characterization of Polyclonal Antibodies Against Human Chaperonin 10

Overview

Journal Cell Stress Chaperones

Publisher Elsevier

Specialty Cell Biology

Date 2000 Mar 4

PMID 10701835

Citations 3

Authors

N C Hillyard

H Morton

D Alewood

J A Halliday

P F Alewood

D A Vesey

M D Walsh

A C Cavanagh

Affiliations

Soon will be listed here.

Abstract

Early pregnancy factor (EPF) has been identified as an extracellular homologue of chaperonin 10 (Cpn10), a heat shock protein that functions within the cell as a molecular chaperone. Here, we report the production of polyclonal antibodies directed against several different regions of the human Cpn10 molecule and their application to specific protein quantitation and localization techniques. These antibodies will be valuable tools in further studies to elucidate the mechanisms underlying the differential spatial and temporal localization of EPF and Cpn10 and in studies to elucidate structure and function.

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References

Morton H, Hegh V, CLUNIE G . Production and assay of dog, pig and goat heterologous anti-rabbit lymphocyte serum. Clin Exp Immunol. 1973; 13(4):595-602. PMC: 1553738. View

Summers K, Murphy R, Webb G, Peters G, Morton H, Cassady A . The human early pregnancy factor/chaperonin 10 gene family. Biochem Mol Med. 1996; 58(1):52-8. DOI: 10.1006/bmme.1996.0032. View

Noonan F, HALLIDAY W, Morton H, CLUNIE G . Early pregnancy factor is immunosuppressive. Nature. 1979; 278(5705):649-51. DOI: 10.1038/278649a0. View

Dunn S . Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on Western blots by monoclonal antibodies. Anal Biochem. 1986; 157(1):144-53. DOI: 10.1016/0003-2697(86)90207-1. View

Morton H, Rolfe B, Cavanagh A . Ovum factor and early pregnancy factor. Curr Top Dev Biol. 1987; 23:73-92. DOI: 10.1016/s0070-2153(08)60620-1. View

Quinn K, Wong T, Rolfe B, Cavanagh A, Morton H . Passive immunization of pregnant mice against early pregnancy factor causes loss of embryonic viability. J Reprod Fertil. 1989; 87(2):495-502. DOI: 10.1530/jrf.0.0870495. View

Quinn K, Wong T, Rolfe B, Cavanagh A, Morton H . Monoclonal antibodies to early pregnancy factor perturb tumour cell growth. Clin Exp Immunol. 1990; 80(1):100-8. PMC: 1535227. DOI: 10.1111/j.1365-2249.1990.tb06448.x. View

Shi S, Key M, Kalra K . Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem. 1991; 39(6):741-8. DOI: 10.1177/39.6.1709656. View

Quinn K . Active immunization with EPF suppresses the formation of immune ascites in BALB/c mice. Immunol Cell Biol. 1991; 69 ( Pt 1):1-6. DOI: 10.1038/icb.1991.1. View

10.

Ellis R, Van Der Vies S . Molecular chaperones. Annu Rev Biochem. 1991; 60:321-47. DOI: 10.1146/annurev.bi.60.070191.001541. View

11.

Morton H, Dunglison G, Kaye P . Antibodies to early pregnancy factor retard embryonic development in mice in vivo. J Reprod Fertil. 1991; 92(2):443-51. DOI: 10.1530/jrf.0.0920443. View

12.

Zeilstra-Ryalls J, Fayet O, Georgopoulos C . The universally conserved GroE (Hsp60) chaperonins. Annu Rev Microbiol. 1991; 45:301-25. DOI: 10.1146/annurev.mi.45.100191.001505. View

13.

Quinn K, Morton H . Effect of monoclonal antibodies to early pregnancy factor (EPF) on the in vivo growth of transplantable murine tumours. Cancer Immunol Immunother. 1992; 34(4):265-71. PMC: 11038521. DOI: 10.1007/BF01741795. View

14.

Hartman D, Hoogenraad N, Condron R, Hoj P . Identification of a mammalian 10-kDa heat shock protein, a mitochondrial chaperonin 10 homologue essential for assisted folding of trimeric ornithine transcarbamoylase in vitro. Proc Natl Acad Sci U S A. 1992; 89(8):3394-8. PMC: 48874. DOI: 10.1073/pnas.89.8.3394. View

15.

Cavanagh A, Morton H . The purification of early-pregnancy factor to homogeneity from human platelets and identification as chaperonin 10. Eur J Biochem. 1994; 222(2):551-60. DOI: 10.1111/j.1432-1033.1994.tb18897.x. View

16.

Quinn K, Cavanagh A, Hillyard N, McKay D, Morton H . Early pregnancy factor in liver regeneration after partial hepatectomy in rats: relationship with chaperonin 10. Hepatology. 1994; 20(5):1294-302. View

17.

Allauzen S, Mani J, Granier C, Pau B, Bouanani M . Epitope mapping and binding analysis of insulin-specific monoclonal antibodies using a biosensor approach. J Immunol Methods. 1995; 183(1):27-32. DOI: 10.1016/0022-1759(95)00020-b. View

18.

Morton H, Hegh V, CLUNIE G . Studies of the rosette inhibition test in pregnant mice: evidence of immunosuppression?. Proc R Soc Lond B Biol Sci. 1976; 193(1113):413-9. DOI: 10.1098/rspb.1976.0054. View