Experimental Inoculation with Human Granulocytic Ehrlichia Agent Derived from High- and Low-passage Cell Culture in Horses

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2000 Mar 4

PMID 10699041

Citations 10

Authors

N Pusterla

J E Madigan

K M Asanovich

J S Chae

E DeRock

C M Leutenegger

J B Pusterla

H Lutz

J S Dumler

Affiliations

Soon will be listed here.

Abstract

We report the successful infection throughout intravenous inoculation with low and high passage of in vitro-grown human granulocytic ehrlichiosis (HGE) agent in horses. Differences in disease severity but not in incubation time, hematological changes, PCR detection, ehrlichial load, seroconversion time, and titer range were noted between horses infected with a low and a high passage of in vitro-grown HGE agent.

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References

Tuomi J . Experimental studies on bovine tick-borne fever. 2. Differences in virulence of strains in cattle and sheep. Acta Pathol Microbiol Scand. 1967; 70(4):577-89. View

Pusterla N, Huder J, Leutenegger C, Braun U, Madigan J, Lutz H . Quantitative real-time PCR for detection of members of the Ehrlichia phagocytophila genogroup in host animals and Ixodes ricinus ticks. J Clin Microbiol. 1999; 37(5):1329-31. PMC: 84766. DOI: 10.1128/JCM.37.5.1329-1331.1999. View

Purnell R, BROCKLESBY D . Isolation of a virulent strain of Ehrlichia phagocytophila from the blood of cattle in the Isle of Man. Vet Rec. 1978; 102(25):552-3. DOI: 10.1136/vr.102.25.552. View

Madigan J, Hietala S, Chalmers S, DeRock E . Seroepidemiologic survey of antibodies to Ehrlichia equi in horses of northern California. J Am Vet Med Assoc. 1990; 196(12):1962-4. View

Jongejan F . Protective immunity to heartwater (Cowdria ruminantium infection) is acquired after vaccination with in vitro-attenuated rickettsiae. Infect Immun. 1991; 59(2):729-31. PMC: 257822. DOI: 10.1128/iai.59.2.729-731.1991. View

Chen S, Dumler J, Bakken J, Walker D . Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J Clin Microbiol. 1994; 32(3):589-95. PMC: 263091. DOI: 10.1128/jcm.32.3.589-595.1994. View

Bakken J, Dumler J, Chen S, Eckman M, Van Etta L, Walker D . Human granulocytic ehrlichiosis in the upper Midwest United States. A new species emerging?. JAMA. 1994; 272(3):212-8. View

Norris S, Howell J, Garza S, Ferdows M, Barbour A . High- and low-infectivity phenotypes of clonal populations of in vitro-cultured Borrelia burgdorferi. Infect Immun. 1995; 63(6):2206-12. PMC: 173287. DOI: 10.1128/iai.63.6.2206-2212.1995. View

Madigan J, Richter Jr P, Kimsey R, Barlough J, Bakken J, Dumler J . Transmission and passage in horses of the agent of human granulocytic ehrlichiosis. J Infect Dis. 1995; 172(4):1141-4. DOI: 10.1093/infdis/172.4.1141. View

10.

Goodman J, Nelson C, Vitale B, Madigan J, Dumler J, Kurtti T . Direct cultivation of the causative agent of human granulocytic ehrlichiosis. N Engl J Med. 1996; 334(4):209-15. DOI: 10.1056/NEJM199601253340401. View

11.

Carroll J, Gherardini F . Membrane protein variations associated with in vitro passage of Borrelia burgdorferi. Infect Immun. 1996; 64(2):392-8. PMC: 173776. DOI: 10.1128/iai.64.2.392-398.1996. View

12.

Barlough J, Madigan J, DeRock E, Dumler J, Bakken J . Protection against Ehrlichia equi is conferred by prior infection with the human granulocytotropic Ehrlichia (HGE agent). J Clin Microbiol. 1995; 33(12):3333-4. PMC: 228702. DOI: 10.1128/jcm.33.12.3333-3334.1995. View

13.

Madigan J, Barlough J, Dumler J, Schankman N, DeRock E . Equine granulocytic ehrlichiosis in Connecticut caused by an agent resembling the human granulocytotropic ehrlichia. J Clin Microbiol. 1996; 34(2):434-5. PMC: 228814. DOI: 10.1128/jcm.34.2.434-435.1996. View

14.

Barlough J, Madigan J, DeRock E, Bigornia L . Nested polymerase chain reaction for detection of Ehrlichia equi genomic DNA in horses and ticks (Ixodes pacificus). Vet Parasitol. 1996; 63(3-4):319-29. DOI: 10.1016/0304-4017(95)00904-3. View

15.

Hu C, Simon M, Kramer M, Gern L . Tick factors and in vitro cultivation influence the protein profile, antigenicity and pathogenicity of a cloned Borrelia garinii isolate from Ixodes ricinus hemolymph. Infection. 1996; 24(3):251-7. DOI: 10.1007/BF01781105. View

16.

Petrovec M, Lotric Furlan S, Zupanc T, Strle F, Brouqui P, Roux V . Human disease in Europe caused by a granulocytic Ehrlichia species. J Clin Microbiol. 1997; 35(6):1556-9. PMC: 229786. DOI: 10.1128/jcm.35.6.1556-1559.1997. View

17.

Asanovich K, Bakken J, Madigan J, Aguero-Rosenfeld M, Wormser G, Dumler J . Antigenic diversity of granulocytic Ehrlichia isolates from humans in Wisconsin and New York and a horse in California. J Infect Dis. 1997; 176(4):1029-34. DOI: 10.1086/516529. View

18.

Fritz C, Glaser C . Ehrlichiosis. Infect Dis Clin North Am. 1998; 12(1):123-36. DOI: 10.1016/s0891-5520(05)70413-x. View

19.

Chang Y, Novosel V, Dubovi E, Wong S, Chu F, Chang C . Experimental infection of the human granulocytic ehrlichiosis agent in horses. Vet Parasitol. 1998; 78(2):137-45. DOI: 10.1016/s0304-4017(98)00133-2. View

20.

Ristic M, Carson C . Methods of immunoprophylaxis against bovine anaplasmosis with emphasis on use of the attenuated Anaplasma marginale vaccine. Adv Exp Med Biol. 1977; 93:151-88. DOI: 10.1007/978-1-4615-8855-9_10. View