Staphylococcal Surface Display of Metal-binding Polyhistidyl Peptides

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2000 Mar 4

PMID 10698802

Citations 17

Authors

P Samuelson

H Wernerus

M Svedberg

S Stahl

Affiliations

Soon will be listed here.

Abstract

Recombinant Staphylococcus xylosus and Staphylococcus carnosus strains were generated with surface-exposed chimeric proteins containing polyhistidyl peptides designed for binding to divalent metal ions. Surface accessibility of the chimeric surface proteins was demonstrated and the chimeric surface proteins were found to be functional in terms of metal binding, since the recombinant staphylococcal cells were shown to have gained Ni(2+)- and Cd(2+)-binding capacity, suggesting that such bacteria could find use in bioremediation of heavy metals. This is, to our knowledge, the first time that recombinant, surface-exposed metal-binding peptides have been expressed on gram-positive bacteria. Potential environmental or biosensor applications for such recombinant staphylococci as biosorbents are discussed.

Citing Articles

Bacillus subtilis surface display technology: applications in bioprocessing and sustainable manufacturing.

Bahrulolum H, Ahmadian G Biotechnol Biofuels Bioprod. 2025; 18(1):34.

PMID: 40089785 DOI: 10.1186/s13068-025-02635-4.

Enhanced biosorption of cadmium ions on immobilized surface-engineered yeast using cadmium-binding peptides.

Wang S, Sun Y, Wang S, Fan C, Wang D, Liu F Front Microbiol. 2024; 15:1496843.

PMID: 39619698 PMC: 11604839. DOI: 10.3389/fmicb.2024.1496843.

Engineered Yeast Displaying Specific Norovirus-Binding Nanobodies for the Concentration and Detection of Human Norovirus in Food Matrix.

Zhao X, Rahman M, Xu Z, Kasputis T, He Y, Yuan L J Agric Food Chem. 2023; 71(22):8665-8672.

PMID: 37227100 PMC: 10332245. DOI: 10.1021/acs.jafc.3c01946.

Recent advances in bacterial biosensing and bioremediation of cadmium pollution: a mini-review.

Hui C, Guo Y, Liu L, Yi J World J Microbiol Biotechnol. 2021; 38(1):9.

PMID: 34850291 DOI: 10.1007/s11274-021-03198-w.

A Novel Cu(II)-Binding Peptide Identified by Phage Display Inhibits Cu-Mediated Aβ Aggregation.

Zhang X, Zhang X, Zhong M, Zhao P, Guo C, Li Y Int J Mol Sci. 2021; 22(13).

PMID: 34202166 PMC: 8269028. DOI: 10.3390/ijms22136842.

References

Sousa C, Kotrba P, Ruml T, Cebolla A, de Lorenzo V . Metalloadsorption by Escherichia coli cells displaying yeast and mammalian metallothioneins anchored to the outer membrane protein LamB. J Bacteriol. 1998; 180(9):2280-4. PMC: 107165. DOI: 10.1128/JB.180.9.2280-2284.1998. View

Patwardhan A, Goud G, Koepsel R, Ataai M . Selection of optimum affinity tags from a phage-displayed peptide library. Application to immobilized copper(II) affinity chromatography. J Chromatogr A. 1997; 787(1-2):91-100. DOI: 10.1016/s0021-9673(97)00580-3. View

Mejare M, Ljung S, Bulow L . Selection of cadmium specific hexapeptides and their expression as OmpA fusion proteins in Escherichia coli. Protein Eng. 1998; 11(6):489-94. DOI: 10.1093/protein/11.6.489. View

Pazirandeh M, Wells B, RYAN R . Development of bacterium-based heavy metal biosorbents: enhanced uptake of cadmium and mercury by Escherichia coli expressing a metal binding motif. Appl Environ Microbiol. 1998; 64(10):4068-72. PMC: 106604. DOI: 10.1128/AEM.64.10.4068-4072.1998. View

Malik P, Terry T, Bellintani F, Perham R . Factors limiting display of foreign peptides on the major coat protein of filamentous bacteriophage capsids and a potential role for leader peptidase. FEBS Lett. 1998; 436(2):263-6. DOI: 10.1016/s0014-5793(98)01140-5. View

Pagan R, Manas P, Raso J, Condon S . Bacterial resistance to ultrasonic waves under pressure at nonlethal (manosonication) and lethal (manothermosonication) temperatures. Appl Environ Microbiol. 1999; 65(1):297-300. PMC: 91018. DOI: 10.1128/AEM.65.1.297-300.1999. View

Schembri M, Kjaergaard K, Klemm P . Bioaccumulation of heavy metals by fimbrial designer adhesins. FEMS Microbiol Lett. 1999; 170(2):363-71. DOI: 10.1111/j.1574-6968.1999.tb13396.x. View

Kotrba P, Doleckova L, de Lorenzo V, Ruml T . Enhanced bioaccumulation of heavy metal ions by bacterial cells due to surface display of short metal binding peptides. Appl Environ Microbiol. 1999; 65(3):1092-8. PMC: 91149. DOI: 10.1128/AEM.65.3.1092-1098.1999. View

Kotrba P, Pospisil P, de Lorenzo V, Ruml T . Enhanced metallosorption of Escherichia coli cells due to surface display of beta- and alpha-domains of mammalian metallothionein as a fusion to LamB protein. J Recept Signal Transduct Res. 1999; 19(1-4):703-15. DOI: 10.3109/10799899909036681. View

10.

Nord K, Gunneriusson E, Ringdahl J, Stahl S, Uhlen M, Nygren P . Binding proteins selected from combinatorial libraries of an alpha-helical bacterial receptor domain. Nat Biotechnol. 1997; 15(8):772-7. DOI: 10.1038/nbt0897-772. View

11.

Kelemen M, SHARPE J . Controlled cell disruption: a comparison of the forces required to disrupt different micro-organisms. J Cell Sci. 1979; 35:431-41. DOI: 10.1242/jcs.35.1.431. View

12.

Gotz F, Ahrne S, Lindberg M . Plasmid transfer and genetic recombination by protoplast fusion in staphylococci. J Bacteriol. 1981; 145(1):74-81. PMC: 217246. DOI: 10.1128/jb.145.1.74-81.1981. View

13.

Uhlen M, Nilsson B, Guss B, Lindberg M, Gatenbeck S, Philipson L . Gene fusion vectors based on the gene for staphylococcal protein A. Gene. 1983; 23(3):369-78. DOI: 10.1016/0378-1119(83)90025-2. View

14.

Guss B, Uhlen M, Nilsson B, Lindberg M, Sjoquist J, Sjodahl J . Region X, the cell-wall-attachment part of staphylococcal protein A. Eur J Biochem. 1984; 138(2):413-20. DOI: 10.1111/j.1432-1033.1984.tb07931.x. View

15.

Hultman T, Stahl S, Hornes E, Uhlen M . Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res. 1989; 17(13):4937-46. PMC: 318085. DOI: 10.1093/nar/17.13.4937. View

16.

Stahl S, Sjolander A, Nygren P, Berzins K, Perlmann P, Uhlen M . A dual expression system for the generation, analysis and purification of antibodies to a repeated sequence of the Plasmodium falciparum antigen Pf155/RESA. J Immunol Methods. 1989; 124(1):43-52. DOI: 10.1016/0022-1759(89)90184-1. View

17.

Ljungquist C, Breitholtz A, Moks T, Uhlen M, Nilsson B . Immobilization and affinity purification of recombinant proteins using histidine peptide fusions. Eur J Biochem. 1989; 186(3):563-9. DOI: 10.1111/j.1432-1033.1989.tb15245.x. View

18.

Mullen M, Wolf D, Ferris F, Beveridge T, Flemming C, Bailey G . Bacterial sorption of heavy metals. Appl Environ Microbiol. 1989; 55(12):3143-9. PMC: 203237. DOI: 10.1128/aem.55.12.3143-3149.1989. View

19.

Nygren P, Eliasson M, Abrahmsen L, Uhlen M, Palmcrantz E . Analysis and use of the serum albumin binding domains of streptococcal protein G. J Mol Recognit. 1988; 1(2):69-74. DOI: 10.1002/jmr.300010204. View

20.

Gotz F . Staphylococcus carnosus: a new host organism for gene cloning and protein production. Soc Appl Bacteriol Symp Ser. 1990; 19:49S-53S. DOI: 10.1111/j.1365-2672.1990.tb01797.x. View