Association of SWAP-70 with the B Cell Antigen Receptor Complex

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2000 Feb 19

PMID 10681448

Citations 21

Authors

L Masat

J Caldwell

R Armstrong

H Khoshnevisan

R Jessberger

B Herndier

M Wabl

D Ferrick

Affiliations

Soon will be listed here.

Abstract

SWAP-70 is a component of an enzyme complex that recombines Ig switch regions in vitro. We report here the cloning of the human cDNA and its B lymphocyte-specific expression. Although its sequence contains three nuclear localization signals, in small resting B cells, SWAP-70 is mainly found in the cytoplasm. On stimulation, SWAP-70 translocates to the nucleus. In activated, class-switching B cell cultures, it is associated with membrane IgG, but not IgM. The membrane Ig association requires a functional pleckstrin homology domain and is controlled by the C terminus. We suggest that SWAP-70 is involved not only in nuclear events but also in signaling in B cell activation.

Citing Articles

GWSF-EuSWAP70 gene expression to enhance gray mold resistance in Arabidopsis thaliana.

Huang Z, Li X, Cai F, Li L, Wu Z BMC Plant Biol. 2025; 25(1):152.

PMID: 39910475 PMC: 11796068. DOI: 10.1186/s12870-024-06002-7.

A spatiotemporal map of co-receptor signaling networks underlying B cell activation.

Susa K, Bradshaw G, Eisert R, Schilling C, Kalocsay M, Blacklow S Cell Rep. 2024; 43(6):114332.

PMID: 38850533 PMC: 11256977. DOI: 10.1016/j.celrep.2024.114332.

B cell phenotypes and maturation states in cows naturally infected with Mycobacterium avium subsp. Paratuberculosis.

Stabel J, Bannantine J, Humphrey S PLoS One. 2022; 17(12):e0278313.

PMID: 36477266 PMC: 9728927. DOI: 10.1371/journal.pone.0278313.

Regulation of systemic autoimmunity and CD11c Tbet B cells by SWEF proteins.

Manni M, Ricker E, Pernis A Cell Immunol. 2017; 321:46-51.

PMID: 28780965 PMC: 5877469. DOI: 10.1016/j.cellimm.2017.05.010.

Identification of a five B cell-associated gene prognostic and predictive signature for advanced glioma patients harboring immunosuppressive subtype preference.

Zhang C, Li J, Wang H, Song S Oncotarget. 2016; 7(45):73971-73983.

PMID: 27738332 PMC: 5342028. DOI: 10.18632/oncotarget.12605.

References

Robbins J, Dilworth S, Laskey R, Dingwall C . Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence. Cell. 1991; 64(3):615-23. DOI: 10.1016/0092-8674(91)90245-t. View

Borggrefe T, Masat L, Wabl M, Riwar B, Cattoretti G, Jessberger R . Cellular, intracellular, and developmental expression patterns of murine SWAP-70. Eur J Immunol. 1999; 29(6):1812-22. DOI: 10.1002/(SICI)1521-4141(199906)29:06<1812::AID-IMMU1812>3.0.CO;2-J. View

Reth M . Antigen receptors on B lymphocytes. Annu Rev Immunol. 1992; 10:97-121. DOI: 10.1146/annurev.iy.10.040192.000525. View

Marchini A, Longnecker R, Kieff E . Epstein-Barr virus (EBV)-negative B-lymphoma cell lines for clonal isolation and replication of EBV recombinants. J Virol. 1992; 66(8):4972-81. PMC: 241347. DOI: 10.1128/JVI.66.8.4972-4981.1992. View

Pear W, Nolan G, Scott M, Baltimore D . Production of high-titer helper-free retroviruses by transient transfection. Proc Natl Acad Sci U S A. 1993; 90(18):8392-6. PMC: 47362. DOI: 10.1073/pnas.90.18.8392. View

Kotani H, Newton 3rd P, Zhang S, Chiang Y, Otto E, Weaver L . Improved methods of retroviral vector transduction and production for gene therapy. Hum Gene Ther. 1994; 5(1):19-28. DOI: 10.1089/hum.1994.5.1-19. View

Macias M, Musacchio A, Ponstingl H, Nilges M, Saraste M, Oschkinat H . Structure of the pleckstrin homology domain from beta-spectrin. Nature. 1994; 369(6482):675-7. DOI: 10.1038/369675a0. View

Pleiman C, DAmbrosio D, Cambier J . The B-cell antigen receptor complex: structure and signal transduction. Immunol Today. 1994; 15(9):393-9. DOI: 10.1016/0167-5699(94)90267-4. View

Rensink H, Rappuoli R, Aarden L . Co-stimulation of T cells via CD28 inhibits human IgE production; reversal by pertussis toxin. Clin Exp Immunol. 1995; 99(3):473-8. PMC: 1534210. DOI: 10.1111/j.1365-2249.1995.tb05575.x. View

10.

Pitcher J, Touhara K, Payne E, Lefkowitz R . Pleckstrin homology domain-mediated membrane association and activation of the beta-adrenergic receptor kinase requires coordinate interaction with G beta gamma subunits and lipid. J Biol Chem. 1995; 270(20):11707-10. DOI: 10.1074/jbc.270.20.11707. View

11.

Onishi M, Kinoshita S, Morikawa Y, Shibuya A, Phillips J, Lanier L . Applications of retrovirus-mediated expression cloning. Exp Hematol. 1996; 24(2):324-9. View

12.

Hicks G, Raikhel N . Protein import into the nucleus: an integrated view. Annu Rev Cell Dev Biol. 1995; 11:155-88. DOI: 10.1146/annurev.cb.11.110195.001103. View

13.

Achatz G, Nitschke L, Lamers M . Effect of transmembrane and cytoplasmic domains of IgE on the IgE response. Science. 1997; 276(5311):409-11. DOI: 10.1126/science.276.5311.409. View

14.

DeFranco A . The complexity of signaling pathways activated by the BCR. Curr Opin Immunol. 1997; 9(3):296-308. DOI: 10.1016/s0952-7915(97)80074-x. View

15.

Casellas R, Nussenzweig A, Wuerffel R, Pelanda R, Reichlin A, Suh H . Ku80 is required for immunoglobulin isotype switching. EMBO J. 1998; 17(8):2404-11. PMC: 1170583. DOI: 10.1093/emboj/17.8.2404. View

16.

Manis J, Gu Y, Lansford R, Sonoda E, Ferrini R, Davidson L . Ku70 is required for late B cell development and immunoglobulin heavy chain class switching. J Exp Med. 1998; 187(12):2081-9. PMC: 2212369. DOI: 10.1084/jem.187.12.2081. View

17.

Taagepera S, McDonald D, Loeb J, Whitaker L, McElroy A, Wang J . Nuclear-cytoplasmic shuttling of C-ABL tyrosine kinase. Proc Natl Acad Sci U S A. 1998; 95(13):7457-62. PMC: 22649. DOI: 10.1073/pnas.95.13.7457. View

18.

Borggrefe T, Wabl M, Akhmedov A, Jessberger R . A B-cell-specific DNA recombination complex. J Biol Chem. 1998; 273(27):17025-35. DOI: 10.1074/jbc.273.27.17025. View

19.

Rebecchi M, Scarlata S . Pleckstrin homology domains: a common fold with diverse functions. Annu Rev Biophys Biomol Struct. 1998; 27:503-28. DOI: 10.1146/annurev.biophys.27.1.503. View

20.

von Schwedler U, Jack H, Wabl M . Beswitched. The looping out model for immunoglobulin class switching. New Biol. 1990; 2(8):657-62. View