Enhancement of Secretion and Extracellular Stability of Staphylokinase in Bacillus Subtilis by WprA Gene Disruption

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2000 Feb 2

PMID 10653706

Citations 5

Authors

S J Lee

D M Kim

K H Bae

S M Byun

J H Chung

Affiliations

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Abstract

Staphylokinase (SAK), a polypeptide secreted by Staphylococcus aureus, is a plasminogen activator with a therapeutic potential in thrombosis diseases. A Bacillus subtilis strain which is multiply deficient in exoproteases was transformed by an expression plasmid carrying a promoter and a signal sequence of subtilisin fused in frame with the sak open reading frame. However, the amount of SAK secretion was marginal (45 mg/liter). In contrast, disruption of the wprA gene, which encodes a subtilisin-type protease, strongly promoted the production of SAK in the stationary phase (181 mg/liter). In addition, the extracellular stability of mature SAK was dramatically enhanced. These data indicate a significant role of the wprA gene product in degrading foreign proteins, both during secretion and in the extracellular milieu.

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