The Common CDNA and Amino Acid Sequences of the CD14 (myeloid Cell-specific Leucine-rich Glycoprotein) Receptor

The CD14 receptor is a myeloid cell specific receptor, which plays a role in the recognition of lipopolysaccharides (endotoxins of gram-negative bacteria) and cell stimulation. To date, several sequences of the cDNA of the CD14 receptor have been described. We sought to establish whether the substitutions C(230)-->G, and G(560)-->A are polymorphic or if they result from a PCR or sequencing error. Using two mismatched PCRs, we confirmed (on 75 unrelated probands) that the substitutions are not due to common polymorphisms. The common cDNA sequence has the C in position 230 and G in position 560. This corresponds to the amino acids Ala and Cys in positions 77 and 187, respectively.