Selection of Functional TRNA Primers and Primer Binding Site Sequences from a Retroviral Combinatorial Library: Identification of New Functional TRNA Primers in Murine Leukemia Virus Replication

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2000 Jan 19

PMID 10637332

Citations 2

Authors

A H Lund

M Duch

F S Pedersen

Affiliations

Soon will be listed here.

Abstract

Retroviral reverse transcription is initiated from a cellular tRNA molecule and all known exogenous isolates of murine leukemia virus utilise a tRNA(Pro)molecule. While several studies suggest flexibility in murine leukemia virus primer utilisation, studies on human immunodeficiency virus and avian retro-viruses have revealed evidence of molecular adapt-ation towards the specific tRNA isoacceptor used as replication primer. In this study, murine leukemia virus tRNA utilisation is investigated by in vivo screening of a retroviral vector combinatorial library with randomised primer binding sites. While most of the selected primer binding sites are complementary to the 3'-end of tRNA((Pro)), we also retrieved PBS sequences matching four other tRNA molecules and demonstrate that Akv murine leukemia virus vectors may efficiently replicate using tRNA(Arg(CCU)), tRNA(Phe(GAA))and a hitherto unknown human tRNA(Ser(CGA)).

Citing Articles

Forced selection of a human immunodeficiency virus type 1 variant that uses a non-self tRNA primer for reverse transcription: involvement of viral RNA sequences and the reverse transcriptase enzyme.

Abbink T, Beerens N, Berkhout B J Virol. 2004; 78(19):10706-14.

PMID: 15367637 PMC: 516392. DOI: 10.1128/JVI.78.19.10706-10714.2004.

tRNASer(CGA) differentially regulates expression of wild-type and codon-modified papillomavirus L1 genes.

Gu W, Li M, Zhao W, Fang N, Bu S, Frazer I Nucleic Acids Res. 2004; 32(15):4448-61.

PMID: 15319446 PMC: 516046. DOI: 10.1093/nar/gkh748.

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