Clathrin: a Unique Protein Associated with Intracellular Transfer of Membrane by Coated Vesicles

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1976 Apr 1

PMID 1063406

Citations 209

Authors

B M Pearse

Affiliations

Soon will be listed here.

Abstract

Coated vesicles have been purified from brain, adrenal medulla, and a nonsecreting lymphoma cell line. A single major protein species, clathrin, with an apparent molecular weight of 180,000, forms the coat of all these vesicles. Peptide mapping suggests that the amino acid sequence of clathrin is conserved, irrespective of tissue or species studied. Coated vesicles of different sizes are found. The coats are constructed with variable numbers of clathrin subunits, arranged in closed networks of hexagons and pentagons. The amount of clathrin in lymphoma cells suggests that coated vesicles transfer substantial amounts of membrane within cells, not necessarily in association with a secretory process.

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