Regulation of Mitotic Homeologous Recombination in Yeast. Functions of Mismatch Repair and Nucleotide Excision Repair Genes

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2000 Jan 11

PMID 10628975

Citations 66

Authors

A Nicholson

M Hendrix

G F Crouse

Affiliations

Soon will be listed here.

Abstract

The Saccharomyces cerevisiae homologs of the bacterial mismatch repair proteins MutS and MutL correct replication errors and prevent recombination between homeologous (nonidentical) sequences. Previously, we demonstrated that Msh2p, Msh3p, and Pms1p regulate recombination between 91% identical inverted repeats, and here use the same substrates to show that Mlh1p and Msh6p have important antirecombination roles. In addition, substrates containing defined types of mismatches (base-base mismatches; 1-, 4-, or 12-nt insertion/deletion loops; or 18-nt palindromes) were used to examine recognition of these mismatches in mitotic recombination intermediates. Msh2p was required for recognition of all types of mismatches, whereas Msh6p recognized only base-base mismatches and 1-nt insertion/deletion loops. Msh3p was involved in recognition of the palindrome and all loops, but also had an unexpected antirecombination role when the potential heteroduplex contained only base-base mismatches. In contrast to their similar antimutator roles, Pms1p consistently inhibited recombination to a lesser degree than did Msh2p. In addition to the yeast MutS and MutL homologs, the exonuclease Exo1p and the nucleotide excision repair proteins Rad1p and Rad10p were found to have roles in inhibiting recombination between mismatched substrates.

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References

Ross-Macdonald P, Roeder G . Mutation of a meiosis-specific MutS homolog decreases crossing over but not mismatch correction. Cell. 1994; 79(6):1069-80. DOI: 10.1016/0092-8674(94)90037-x. View

Habraken Y, Sung P, Prakash L, Prakash S . Binding of insertion/deletion DNA mismatches by the heterodimer of yeast mismatch repair proteins MSH2 and MSH3. Curr Biol. 1996; 6(9):1185-7. DOI: 10.1016/s0960-9822(02)70686-6. View

Sweder K . Nucleotide excision repair in yeast. Curr Genet. 1994; 27(1):1-16. DOI: 10.1007/BF00326572. View

Selva E, New L, Crouse G, Lahue R . Mismatch correction acts as a barrier to homeologous recombination in Saccharomyces cerevisiae. Genetics. 1995; 139(3):1175-88. PMC: 1206448. DOI: 10.1093/genetics/139.3.1175. View

Datta A . Association of increased spontaneous mutation rates with high levels of transcription in yeast. Science. 1995; 268(5217):1616-9. DOI: 10.1126/science.7777859. View

Hollingsworth N, Ponte L, Halsey C . MSH5, a novel MutS homolog, facilitates meiotic reciprocal recombination between homologs in Saccharomyces cerevisiae but not mismatch repair. Genes Dev. 1995; 9(14):1728-39. DOI: 10.1101/gad.9.14.1728. View

de Wind N, Dekker M, Berns A, Radman M, Te Riele H . Inactivation of the mouse Msh2 gene results in mismatch repair deficiency, methylation tolerance, hyperrecombination, and predisposition to cancer. Cell. 1995; 82(2):321-30. DOI: 10.1016/0092-8674(95)90319-4. View

Ito H, Fukuda Y, Murata K, Kimura A . Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983; 153(1):163-8. PMC: 217353. DOI: 10.1128/jb.153.1.163-168.1983. View

Higgins D, Prakash S, Reynolds P, Prakash L . Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae. Gene. 1983; 26(2-3):119-26. DOI: 10.1016/0378-1119(83)90181-6. View

10.

Marsh J, Erfle M, Wykes E . The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene. 1984; 32(3):481-5. DOI: 10.1016/0378-1119(84)90022-2. View

11.

Weiss W, Friedberg E . Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli. EMBO J. 1985; 4(6):1575-82. PMC: 554384. DOI: 10.1002/j.1460-2075.1985.tb03819.x. View

12.

Alani E, Cao L, Kleckner N . A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics. 1987; 116(4):541-5. PMC: 1203166. DOI: 10.1534/genetics.112.541.test. View

13.

Chambers S, Prior S, Barstow D, Minton N . The pMTL nic- cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing. Gene. 1988; 68(1):139-49. DOI: 10.1016/0378-1119(88)90606-3. View

14.

Sikorski R, Hieter P . A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 1989; 122(1):19-27. PMC: 1203683. DOI: 10.1093/genetics/122.1.19. View

15.

Rayssiguier C, Thaler D, Radman M . The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants. Nature. 1989; 342(6248):396-401. DOI: 10.1038/342396a0. View

16.

Nag D, Petes T . Seven-base-pair inverted repeats in DNA form stable hairpins in vivo in Saccharomyces cerevisiae. Genetics. 1991; 129(3):669-73. PMC: 1204734. DOI: 10.1093/genetics/129.3.669. View

17.

Bardwell L, Cooper A, Friedberg E . Stable and specific association between the yeast recombination and DNA repair proteins RAD1 and RAD10 in vitro. Mol Cell Biol. 1992; 12(7):3041-9. PMC: 364518. DOI: 10.1128/mcb.12.7.3041-3049.1992. View

18.

Modrich P, Lahue R . Mismatch repair in replication fidelity, genetic recombination, and cancer biology. Annu Rev Biochem. 1996; 65:101-33. DOI: 10.1146/annurev.bi.65.070196.000533. View

19.

Kramer W, Fartmann B, Ringbeck E . Transcription of mutS and mutL-homologous genes in Saccharomyces cerevisiae during the cell cycle. Mol Gen Genet. 1996; 252(3):275-83. DOI: 10.1007/BF02173773. View

20.

Saparbaev M, Prakash L, Prakash S . Requirement of mismatch repair genes MSH2 and MSH3 in the RAD1-RAD10 pathway of mitotic recombination in Saccharomyces cerevisiae. Genetics. 1996; 142(3):727-36. PMC: 1207014. DOI: 10.1093/genetics/142.3.727. View