The MinC Component of the Division Site Selection System in Escherichia Coli Interacts with FtsZ to Prevent Polymerization

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1999 Dec 28

PMID 10611296

Citations 170

Authors

Z Hu

A Mukherjee

S Pichoff

J Lutkenhaus

Affiliations

Soon will be listed here.

Abstract

Positioning of the Z ring at the midcell site in Escherichia coli is assured by the min system, which masks polar sites through topological regulation of MinC, an inhibitor of division. To study how MinC inhibits division, we have generated a MalE-MinC fusion that retains full biological activity. We find that MalE-MinC interacts with FtsZ and prevents polymerization without inhibiting FtsZ's GTPase activity. MalE-MinC19 has reduced ability to inhibit division, reduced affinity for FtsZ, and reduced ability to inhibit FtsZ polymerization. These results, along with MinC localization, suggest that MinC rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings.

Citing Articles

Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves.

Takada S, Fujiwara K Biophys Physicobiol. 2025; 21(4):e210022.

PMID: 39963599 PMC: 11830476. DOI: 10.2142/biophysico.bppb-v21.0022.

Minimization of the Bacillus subtilis divisome suggests FtsZ and SepF can form an active Z-ring, and reveals the amino acid transporter BraB as a new cell division influencing factor.

Gulsoy I, Saaki T, Wenzel M, Syvertsson S, Morimoto T, Siersma T PLoS Genet. 2025; 21(1):e1011567.

PMID: 39869651 PMC: 11790237. DOI: 10.1371/journal.pgen.1011567.

Growth-dependent concentration gradient of the oscillating Min system in Escherichia coli.

Parada C, Yan C, Hung C, Tu I, Hsu C, Shih Y J Cell Biol. 2024; 224(2).

PMID: 39621132 PMC: 11613459. DOI: 10.1083/jcb.202406107.

Septal wall synthesis is sufficient to change ameba-like cells into uniform oval-shaped cells in Escherichia coli L-forms.

Hayashi M, Takaoka C, Higashi K, Kurokawa K, Margolin W, Oshima T Commun Biol. 2024; 7(1):1569.

PMID: 39587276 PMC: 11589767. DOI: 10.1038/s42003-024-07279-y.

Building the Bacterial Divisome at the Septum.

Morrison J, Camberg J Subcell Biochem. 2024; 104():49-71.

PMID: 38963483 DOI: 10.1007/978-3-031-58843-3_4.

References

Raskin D, de Boer P . The MinE ring: an FtsZ-independent cell structure required for selection of the correct division site in E. coli. Cell. 1997; 91(5):685-94. DOI: 10.1016/s0092-8674(00)80455-9. View

Trusca D, Scott S, Thompson C, Bramhill D . Bacterial SOS checkpoint protein SulA inhibits polymerization of purified FtsZ cell division protein. J Bacteriol. 1998; 180(15):3946-53. PMC: 107380. DOI: 10.1128/JB.180.15.3946-3953.1998. View

Bi E, Lutkenhaus J . Interaction between the min locus and ftsZ. J Bacteriol. 1990; 172(10):5610-6. PMC: 526872. DOI: 10.1128/jb.172.10.5610-5616.1990. View

Erickson H, Taylor D, Taylor K, Bramhill D . Bacterial cell division protein FtsZ assembles into protofilament sheets and minirings, structural homologs of tubulin polymers. Proc Natl Acad Sci U S A. 1996; 93(1):519-23. PMC: 40269. DOI: 10.1073/pnas.93.1.519. View

Addinall S, Lutkenhaus J . FtsZ-spirals and -arcs determine the shape of the invaginating septa in some mutants of Escherichia coli. Mol Microbiol. 1996; 22(2):231-7. DOI: 10.1046/j.1365-2958.1996.00100.x. View

Blanchoin L, Pollard T . Interaction of actin monomers with Acanthamoeba actophorin (ADF/cofilin) and profilin. J Biol Chem. 1998; 273(39):25106-11. DOI: 10.1074/jbc.273.39.25106. View

Huang J, Cao C, Lutkenhaus J . Interaction between FtsZ and inhibitors of cell division. J Bacteriol. 1996; 178(17):5080-5. PMC: 178301. DOI: 10.1128/jb.178.17.5080-5085.1996. View

Guzman L, Belin D, CARSON M, Beckwith J . Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995; 177(14):4121-30. PMC: 177145. DOI: 10.1128/jb.177.14.4121-4130.1995. View

Adler H, Fisher W, Cohen A, Hardigree A . MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967; 57(2):321-6. PMC: 335508. DOI: 10.1073/pnas.57.2.321. View

10.

de Boer P, Crossley R, Rothfield L . Central role for the Escherichia coli minC gene product in two different cell division-inhibition systems. Proc Natl Acad Sci U S A. 1990; 87(3):1129-33. PMC: 53424. DOI: 10.1073/pnas.87.3.1129. View

11.

Mukherjee A, Lutkenhaus J . Dynamic assembly of FtsZ regulated by GTP hydrolysis. EMBO J. 1998; 17(2):462-9. PMC: 1170397. DOI: 10.1093/emboj/17.2.462. View

12.

Carlier M, Laurent V, Santolini J, Melki R, Didry D, Xia G . Actin depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover: implication in actin-based motility. J Cell Biol. 1997; 136(6):1307-22. PMC: 2132522. DOI: 10.1083/jcb.136.6.1307. View

13.

Marston A, Thomaides H, Edwards D, Sharpe M, Errington J . Polar localization of the MinD protein of Bacillus subtilis and its role in selection of the mid-cell division site. Genes Dev. 1998; 12(21):3419-30. PMC: 317235. DOI: 10.1101/gad.12.21.3419. View

14.

Ward Jr J, Lutkenhaus J . Overproduction of FtsZ induces minicell formation in E. coli. Cell. 1985; 42(3):941-9. DOI: 10.1016/0092-8674(85)90290-9. View

15.

Labie C, BOUCHE F, Bouche J . Minicell-forming mutants of Escherichia coli: suppression of both DicB- and MinD-dependent division inhibition by inactivation of the minC gene product. J Bacteriol. 1990; 172(10):5852-5. PMC: 526903. DOI: 10.1128/jb.172.10.5852-5855.1990. View

16.

Bi E, Lutkenhaus J . FtsZ ring structure associated with division in Escherichia coli. Nature. 1991; 354(6349):161-4. DOI: 10.1038/354161a0. View

17.

Mukherjee A, Cao C, Lutkenhaus J . Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli. Proc Natl Acad Sci U S A. 1998; 95(6):2885-90. PMC: 19664. DOI: 10.1073/pnas.95.6.2885. View

18.

Rothfield L, Justice S . Bacterial cell division: the cycle of the ring. Cell. 1997; 88(5):581-4. DOI: 10.1016/s0092-8674(00)81899-1. View

19.

Hu Z, Lutkenhaus J . Topological regulation of cell division in Escherichia coli involves rapid pole to pole oscillation of the division inhibitor MinC under the control of MinD and MinE. Mol Microbiol. 1999; 34(1):82-90. DOI: 10.1046/j.1365-2958.1999.01575.x. View

20.

Lu C, Stricker J, Erickson H . FtsZ from Escherichia coli, Azotobacter vinelandii, and Thermotoga maritima--quantitation, GTP hydrolysis, and assembly. Cell Motil Cytoskeleton. 1998; 40(1):71-86. DOI: 10.1002/(SICI)1097-0169(1998)40:1<71::AID-CM7>3.0.CO;2-I. View