Increased Serum Levels of Soluble L-selectin (CD62L) in Patients with Active Systemic Lupus Erythematosus (SLE)

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1999 Dec 22

PMID 10606979

Citations 17

Authors

J Font

P Pizcueta

M Ramos-Casals

R Cervera

M Garcia-Carrasco

M Navarro

M Ingelmo

P Engel

Affiliations

Soon will be listed here.

Abstract

The adhesion molecule L-selectin (CD62L) mediates lymphocyte recirculation and leucocyte rolling on vascular endothelium at sites of inflammation. Serum levels of soluble L-selectin (sL-selectin) were measured in patients with SLE in order to relate these levels to clinical activity and immunological parameters. An ELISA was used to detect the soluble form of human L-selectin (CD62L) in 42 patients with SLE and in 33 healthy individuals. The mean +/- s.e.m. values of sL-selectin were 1285 +/- 121 ng/ml for patients with SLE and 986 +/- 180 ng/ml for healthy blood donors, but there was no significant difference. When patients with active SLE were analysed, higher levels of circulating sL-selectin were found when compared with patients without activity (1497 +/- 167 ng/ml versus 941 +/- 150 ng/ml; P = 0.028). We found a significant correlation between the levels of sL-selectin and of dsDNA antibodies (r = 0.36, P = 0. 044) and between levels of sL-selectin and SLE Disease Activity Index (SLEDAI) score (r = 0.42, P = 0.003). Patients with active SLE studied cross-sectionally showed significant elevations of sL-selectin (CD62L) compared with controls. Thus, the levels of this soluble adhesion molecule correlated with active disease and levels of anti-dsDNA antibodies.

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References

Kanekura S, Kitajima I, Nishi J, Yoshinaga M, Miyata K, Maruyama I . Low shed L-selectin levels in Kawasaki disease with coronary artery lesions: comment on the article by Furukawa et al. Arthritis Rheum. 1996; 39(3):534. DOI: 10.1002/art.1780390323. View

Brandt J, Triplett D, ALVING B, Scharrer I . Criteria for the diagnosis of lupus anticoagulants: an update. On behalf of the Subcommittee on Lupus Anticoagulant/Antiphospholipid Antibody of the Scientific and Standardisation Committee of the ISTH. Thromb Haemost. 1995; 74(4):1185-90. View

Steeber D, Engel P, Miller A, Sheetz M, Tedder T . Ligation of L-selectin through conserved regions within the lectin domain activates signal transduction pathways and integrin function in human, mouse, and rat leukocytes. J Immunol. 1997; 159(2):952-63. View

Cush J, Rothlein R, Lindsley H, Mainolfi E, Lipsky P . Increased levels of circulating intercellular adhesion molecule 1 in the sera of patients with rheumatoid arthritis. Arthritis Rheum. 1993; 36(8):1098-102. DOI: 10.1002/art.1780360810. View

Springer T . Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell. 1994; 76(2):301-14. DOI: 10.1016/0092-8674(94)90337-9. View

Belmont H, Buyon J, Giorno R, Abramson S . Up-regulation of endothelial cell adhesion molecules characterizes disease activity in systemic lupus erythematosus. The Shwartzman phenomenon revisited. Arthritis Rheum. 1994; 37(3):376-83. DOI: 10.1002/art.1780370311. View

Stegeman C, Tervaert J, Huitema M, de Jong P, Kallenberg C . Serum levels of soluble adhesion molecules intercellular adhesion molecule 1, vascular cell adhesion molecule 1, and E-selectin in patients with Wegener's granulomatosis. Relationship to disease activity and relevance during followup. Arthritis Rheum. 1994; 37(8):1228-35. DOI: 10.1002/art.1780370818. View

Tan E, Cohen A, Fries J, MASI A, McShane D, Rothfield N . The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982; 25(11):1271-7. DOI: 10.1002/art.1780251101. View

Gharavi A, Harris E, Asherson R, Hughes G . Anticardiolipin antibodies: isotype distribution and phospholipid specificity. Ann Rheum Dis. 1987; 46(1):1-6. PMC: 1002050. DOI: 10.1136/ard.46.1.1. View

10.

Harris E, Gharavi A, Patel S, Hughes G . Evaluation of the anti-cardiolipin antibody test: report of an international workshop held 4 April 1986. Clin Exp Immunol. 1987; 68(1):215-22. PMC: 1542695. View

11.

Cervera R, Font J, Lopez-Soto A, Casals F, Pallares L, Bove A . Isotype distribution of anticardiolipin antibodies in systemic lupus erythematosus: prospective analysis of a series of 100 patients. Ann Rheum Dis. 1990; 49(2):109-13. PMC: 1003989. DOI: 10.1136/ard.49.2.109. View

12.

Springer T . Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-34. DOI: 10.1038/346425a0. View

13.

Bombardier C, Gladman D, Urowitz M, Caron D, Chang C . Derivation of the SLEDAI. A disease activity index for lupus patients. The Committee on Prognosis Studies in SLE. Arthritis Rheum. 1992; 35(6):630-40. DOI: 10.1002/art.1780350606. View

14.

Spertini O, Schleiffenbaum B, Ruiz Jr P, Tedder T . ELISA for quantitation of L-selectin shed from leukocytes in vivo. J Immunol Methods. 1992; 156(1):115-23. DOI: 10.1016/0022-1759(92)90017-n. View

15.

Cronstein B, Kimmel S, Levin R, Martiniuk F, Weissmann G . A mechanism for the antiinflammatory effects of corticosteroids: the glucocorticoid receptor regulates leukocyte adhesion to endothelial cells and expression of endothelial-leukocyte adhesion molecule 1 and intercellular adhesion molecule 1. Proc Natl Acad Sci U S A. 1992; 89(21):9991-5. PMC: 50263. DOI: 10.1073/pnas.89.21.9991. View

16.

Mason J, Kapahi P, Haskard D . Detection of increased levels of circulating intercellular adhesion molecule 1 in some patients with rheumatoid arthritis but not in patients with systemic lupus erythematosus. Lack of correlation with levels of circulating vascular cell adhesion.... Arthritis Rheum. 1993; 36(4):519-27. DOI: 10.1002/art.1780360412. View

17.

Cervera R, Khamashta M, Font J, Sebastiani G, Gil A, Lavilla P . Systemic lupus erythematosus: clinical and immunologic patterns of disease expression in a cohort of 1,000 patients. The European Working Party on Systemic Lupus Erythematosus. Medicine (Baltimore). 1993; 72(2):113-24. View

18.

Heufelder A, Bahn R . Soluble intercellular adhesion molecule-1 (sICAM-1) in sera of patients with Graves' ophthalmopathy and thyroid diseases. Clin Exp Immunol. 1993; 92(2):296-302. PMC: 1554796. DOI: 10.1111/j.1365-2249.1993.tb03395.x. View

19.

Machold K, Kiener H, Graninger W, Graninger W . Soluble intercellular adhesion molecule-1 (sICAM-1) in patients with rheumatoid arthritis and systemic lupus erythematosus. Clin Immunol Immunopathol. 1993; 68(1):74-8. DOI: 10.1006/clin.1993.1098. View

20.

Carson C, Beall L, Hunder G, Johnson C, Newman W . Serum ELAM-1 is increased in vasculitis, scleroderma, and systemic lupus erythematosus. J Rheumatol. 1993; 20(5):809-14. View