E2F is Required to Prevent Inappropriate S-phase Entry of Mammalian Cells

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1999 Dec 14

PMID 10594038

Citations 14

Authors

S He

B L Cook

B E Deverman

U Weihe

F Zhang

V Prachand

J Zheng

S J Weintraub

Affiliations

Soon will be listed here.

Abstract

E2F is a family of transcription factors that regulates the cell cycle. It is widely accepted that E2F-mediated transactivation of a set of genes is the critical activity that governs cellular progression through G(1) into S phase. In contrast to this hypothesis, we demonstrate that E2F actually suppresses the onset of S phase in two cell types when the cells are arrested by gamma irradiation. Our findings indicate that in these cells, the critical event triggering progression from G(0)/G(1) arrest into S phase is the release of E2F-mediated transrepression of cell cycle genes, not transactivation by E2F. Furthermore, our data suggest that E2F-mediated transactivation is not necessary for the G(1)/S-phase transition in these cells.

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