Establishment of the PCR System Specific to Salmonella Spp. and Its Application for the Inspection of Food and Fecal Samples

Overview

Journal J Vet Med Sci

Specialty Veterinary Medicine

Date 1999 Dec 11

PMID 10593584

Citations 21

Authors

S Makino

H Kurazono

M Chongsanguam

H Hayashi

H Cheun

S Suzuki

T Shirahata

Affiliations

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Abstract

We established the PCR detection system specific to Salmonella species using Salmonella enterotoxin gene (stn). The detection limit was one bacterial cell per one gram of fecal and minced-meat samples using enrichment procedure by Tripticase soy broth or Salmonella enrichment broth, respectively. We concluded that this PCR system is useful for the practical application in the field of the public hygiene.

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