Headgroup Conformation and Lipid--cholesterol Association in Phosphatidylcholine Vesicles: a 31P(1H) Nuclear Overhauser Effect Study

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1975 Sep 1

PMID 1059134

Citations 39

Authors

P L Yeagle

W C Hutton

C H Huang

R B Martin

Affiliations

Soon will be listed here.

Abstract

The nuclear Overhauser effect has been observed in the nuclear magnetic resonance spectra of 31P. The information content of the nuclear Overhauser effect has been applied to the structure and dynamic properties of phosphatidylcholine vesicles. In the vesicles only 1/3 of the theoretical maximum nuclear Overhauser effect enhancement is observed. This result is accounted for by dipolar interactions between the N-methyl protons and the phosphate of phosphatidylcholine, and a correlation time for internal motion of 1.4 X 10(-9) sec. Addition of up to 30% cholesterol does not change the nuclear Overhauser effect enhancement or spin-lattice relaxation time of the vesicles. It is argued that the OH group of cholesterol is hydrogen bonded to the ester carbonyl oxygen of the phosphatidylcholine molecules.

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